Advertisement

Biotechnology Letters

, Volume 31, Issue 3, pp 329–335 | Cite as

Introducing pulsed low-intensity ultrasound to culturing human umbilical cord-derived mesenchymal stem cells

  • Jong Hyun Yoon
  • Eun Youn Roh
  • Sue ShinEmail author
  • Nam Hee Jung
  • Eun Young Song
  • Dong Soon Lee
  • Kyou Sup Han
  • Joung Sung Kim
  • Byoung Jae Kim
  • Hye Won Jeon
  • Kang Sup Yoon
Original Research Paper

Abstract

The human umbilical cord (hUC) is a source of adult tissue-derived mesenchymal stem cells (MSCs). A pulsed low-intensity ultrasound (PLIUS) method is described for increasing the yield of MSCs from whole hUC without enzymatic digestion or growth factor supplementation. Analysis of the immunophenotype of cells and a differentiation study were performed to show the compatibility of MSCs. The mean number of cells recovered from primocultures of hUC was 6 × 105 cells/cm. PLIUS resulted in a 3.3-fold increase in MSC yield at passage 0. PLIUS exposure increases the yield of hUC-MSCs by promoting release and enhancing proliferation.

Keywords

Enzyme digestion Growth factor Human umbilical cord Mesenchymal stem cells Pulsed low-intensity ultrasound 

Notes

Acknowledgments

This study was supported by a grant from Seoul Research and Business Development Program (10548).

Supplementary material

10529_2008_9872_MOESM1_ESM.doc (522 kb)
MOESM1 (DOC 28 kb)

References

  1. Covas DT, Siufi JL, Silva AR, Orellana MD (2003) Isolation and culture of umbilical vein mesenchymal stem cells. Braz J Med Biol Res 36:1179–1183PubMedCrossRefGoogle Scholar
  2. Doan N, Reher P, Meghji S, Harris M (1999) In vitro effects of therapeutic ultrasound on cell proliferation, protein synthesis, and cytokine production by human fibroblasts, osteoblasts, and monocytes. J Oral Maxillofac Surg 57: 409–419; discussion 420Google Scholar
  3. Erices A, Conget P, Minguell JJ (2000) Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol 109:235–242PubMedCrossRefGoogle Scholar
  4. Karahuseyinoglu S, Cinar O, Kilic E, Kara F, Akay GG, Demiralp DO, Tukun A, Uckan D, Can A (2007) Biology of stem cells in human umbilical cord stroma: in situ and in vitro surveys. Stem Cells 25:319–331PubMedCrossRefGoogle Scholar
  5. Lu LL, Liu YJ, Yang SG, Zhao QJ, Wang X, Gong W, Han ZB, Xu ZS, Lu YX, Liu D (2006) Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials. Haematologica 91:1017–1026PubMedGoogle Scholar
  6. Mareschi K, Ferrero I, Rustichelli D, Aschero S, Gammaitoni L, Aglietta M, Madon E, Fagioli F (2006) Expansion of mesenchymal stem cells isolated from pediatric and adult donor bone marrow. J Cell Biochem 97:744–754PubMedCrossRefGoogle Scholar
  7. Min BH, Woo JI, Cho HS, Choi BH, Park SJ, Choi MJ, Park SR (2006) Effects of low-intensity ultrasound (LIUS) stimulation on human cartilage explants. Scand J Rheumatol 35:305–311PubMedCrossRefGoogle Scholar
  8. Mitchell KE, Weiss ML, Mitchell BM, Martin P, Davis D, Morales L, Helwig B, Beerenstrauch M, Abou-Easa K, Hildreth T (2003) Matrix cells from Wharton’s jelly form neurons and glia. Stem Cells 21:50–60PubMedGoogle Scholar
  9. Nanaev AK, Kohnen G, Milovanov AP, Domogatsky SP, Kaufmann P (1997) Stromal differentiation and architecture of the human umbilical cord. Placenta 18:53–64PubMedCrossRefGoogle Scholar
  10. Panepucci RA, Siufi JL, Silva WA Jr, Proto-Siquiera R, Neder L, Orellana M, Rocha V, Covas DT, Zago MA (2004) Comparison of gene expression of umbilical cord vein and bone marrow-derived mesenchymal stem cells. Stem Cells 22:1263–1278PubMedCrossRefGoogle Scholar
  11. Pilla AA, Mont MA, Nasser PR, Khan SA, Figueiredo M, Kaufman JJ, Siffert RS (1990) Non-invasive low-intensity pulsed ultrasound accelerates bone healing in the rabbit. J Orthop trauma 4:246–253PubMedCrossRefGoogle Scholar
  12. Sarugaser R, Lickorish D, Baksh D, Hosseini MM, Davies JE (2005) Human umbilical cord perivascular (HUCPV) cells: a source of mesenchymal progenitors. Stem Cells 23:220–229PubMedCrossRefGoogle Scholar
  13. Seshareddy K, Troyer D, Weiss ML (2008) Method to isolate mesenchymal-like cells from Wharton’s Jelly of umbilical cord. Methods Cell Biol 86:101–119PubMedCrossRefGoogle Scholar
  14. Sobolewski K, Malkowski A, Bankowski E, Jaworski S (2005) Wharton’s jelly as a reservoir of peptide growth factors. Placenta 26:747–752PubMedCrossRefGoogle Scholar
  15. Weiss ML, Medicetty S, Bledsoe AR, Rachakatla RS, Choi M, Merchav S, Luo Y, Rao MS, Velagaleti G, Troyer D (2006) Human umbilical cord matrix stem cells: preliminary characterization and effect of transplantation in a rodent model of Parkinson’s disease. Stem Cells 24:781–792PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Jong Hyun Yoon
    • 1
  • Eun Youn Roh
    • 1
  • Sue Shin
    • 1
    Email author
  • Nam Hee Jung
    • 2
  • Eun Young Song
    • 3
  • Dong Soon Lee
    • 3
  • Kyou Sup Han
    • 3
  • Joung Sung Kim
    • 4
  • Byoung Jae Kim
    • 5
  • Hye Won Jeon
    • 5
  • Kang Sup Yoon
    • 6
  1. 1.Department of Laboratory MedicineSeoul National University Boramae HospitalSeoulSouth Korea
  2. 2.Seoul Metropolitan Public Cord Blood Bank (Allcord)SeoulSouth Korea
  3. 3.Department of Laboratory MedicineSeoul National University College of MedicineSeoulSouth Korea
  4. 4.Department of Family MedicineSeoul National University Boramae HospitalSeoulSouth Korea
  5. 5.Department of Obstetrics and GynecologySeoul National University Boramae HospitalSeoulSouth Korea
  6. 6.Department of Orthopedic SurgerySeoul National University Boramae HospitalSeoulSouth Korea

Personalised recommendations