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Biotechnology Letters

, Volume 31, Issue 3, pp 395–401 | Cite as

Functional expression of the lipase gene Lip2 of Pleurotus sapidus in Escherichia coli

  • Kateryna Zelena
  • Sven Krügener
  • Stefan Lunkenbein
  • Holger Zorn
  • Ralf G. Berger
Original Research Paper

Abstract

The lipase Lip2 of the edible basidiomycete, Pleurotus sapidus, is an extracellular enzyme capable of hydrolysing xanthophyll esters with high efficiency. The gene encoding Lip2 was expressed in Escherichia coli TOP10 using the gene III signal sequence to accumulate proteins in the periplasmatic space. The heterologous expression under control of the araBAD promoter led to the high level production of recombinant protein, mainly as inclusion bodies, but partially in a soluble and active form. A fusion with a C-terminal His tag was used for purification and immunochemical detection of the target protein. This is the first example of a heterologous expression and periplasmatic accumulation of a catalytically active lipase from a basidiomycete fungus.

Keywords

Basidiomycete Escherichia coli Heterologous expression Lipase Xanthophyll esters 

Notes

Acknowledgement

Support of the work by the Deutsche Bundesstiftung Umwelt (AZ 13164) is gratefully acknowledged.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Kateryna Zelena
    • 1
  • Sven Krügener
    • 1
  • Stefan Lunkenbein
    • 1
  • Holger Zorn
    • 2
  • Ralf G. Berger
    • 1
  1. 1.Institut für LebensmittelchemieZentrum Angewandte Chemie der Leibniz Universität HannoverHannoverGermany
  2. 2.Fakultät Bio- und Chemieingenieurwesen, Technische BiochemieTechnische Universität DortmundDortmundGermany

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