Biotechnology Letters

, Volume 30, Issue 12, pp 2105–2110 | Cite as

A refinery sludge deposition site: presence of nahH and alkJ genes and crude oil biodegradation ability of bacterial isolates

  • Nikolaos Arvanitis
  • Efstathios A. Katsifas
  • Kalliopi I. Chalkou
  • Christos Meintanis
  • Amalia D. Karagouni
Original Research Paper


204 bacterial isolates from four Greek refinery sludge deposition sites were investigated for the presence of nahH and alkJ genes encoding key enzymes of both aromatic and aliphatic hydrocarbon degradation pathways by PCR and DNA hybridisation. Members of Pseudomonas, Acinetobacter, Bacillus, Rhodococcus and Arthrobacter play important role in bioremediation processes in sandy/loam soil contaminated with oil and nahH and alkJ genes were present in the 73% of the isolates. Consortia of bacterial isolates that were used for biodegradation of aliphatic and aromatic hydrocarbons in crude oil using liquid cultures exhibited rates from 35% to 48% within 10 days of incubation.


Bacteria Biodegradation Genes for hydrocarbon dehydration (nahH and alkJ) Hydrocarbons 



The authors acknowledge partial funding of this work from the European Community (Grant ACTAPHARM, QLRT-2000-01783).

Supplementary material

10529_2008_9816_MOESM1_ESM.doc (42 kb)
(DOC 42 kb)


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Nikolaos Arvanitis
    • 1
  • Efstathios A. Katsifas
    • 1
  • Kalliopi I. Chalkou
    • 1
  • Christos Meintanis
    • 1
  • Amalia D. Karagouni
    • 1
  1. 1.Faculty of Biology, Department of Botany, Microbiology GroupNational and Kapodistrian University of AthensAthensGreece

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