Over-expression of OsDREB genes lead to enhanced drought tolerance in rice
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The DREB transcription factors, which specifically interact with C-repeat/DRE (A/GCCGAC), play an important role in plant abiotic stress tolerance by controlling the expression of many cold or/and drought-inducible genes in an ABA-independent pathway. We have isolated three novel rice DREB genes, OsDREB1E, OsDREB1G, and OsDREB2B, which are homologous to Arabidopsis DREB genes. The yeast one-hybrid assay indicated that OsDREB1E, OsDREB1G, and OsDREB2B can specifically bind to the C-repeat/DRE element. To elucidate the function of respective OsDREB genes, we have stably introduced these to rice by Agrobacterium-mediated transformation. Transgenic rice plants analysis revealed that over-expression of OsDREB1G and OsDREB2B in rice significantly improved their tolerance to water deficit stress, while over-expression of OsDREB1E could only slightly improved the tolerance to water deficit stress, suggesting that the OsDREBs might participate in the stress response pathway in different manners.
KeywordsDrought OsDREB Rice Transcription factors
The authors thank Dr Fan Chen (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) for providing the vectors of pHisi-1 and pLacZi. We also thank Dr. Junli Zhou and Dr. Chengcai Chu for critical reading of the manuscript. This research is supported by a grant of Chinese National Nature Science Foundation (30770211) and by the 863 High-tech Project from the Ministry of Science and Technology of China (2007AA10Z185).
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