Biotechnology Letters

, Volume 30, Issue 11, pp 1893–1899 | Cite as

Comparison of acute responses of mice livers to short-term exposure to nano-sized or micro-sized silver particles

  • Kyungeun Cha
  • Hye-Won Hong
  • Yeon-Gil Choi
  • Min Joo Lee
  • Jong Hoon Park
  • Hee-Kwon Chae
  • Gyuha Ryu
  • Heejoon Myung
Original Research Paper

Abstract

Mice were fed either 13 nm silver nanoparticles or 2–3.5 μm silver microparticles. The livers were then obtained after 3 days and subjected to a histopathological analysis. The nanoparticle-fed and microparticle-fed livers both exhibited lymphocyte infiltration in the histopathological analysis, suggesting the induction of inflammation. In vitro, a human hepatoma cell line (Huh-7) was treated with the same silver nanoparticles and microparticles. The mitochondrial activity and glutathione production were hardly affected. However, the DNA contents decreased 15% in the nanoparticle-treated cells and 10% in the microparticle-treated cell, suggesting a more potent induction of apoptosis by the nanoparticles. From a microarray analysis of the RNA from the livers of the nano- and micro-particle-fed mice, the expression of genes related to apoptosis and inflammation was found to be altered. These gene expression changes in the nanoparticle-treated livers lead to phenotypical changes, reflecting increased apoptosis and inflammation. The changes in the gene expression were confirmed by using a semi-quantitative RT-PCR.

Keywords

Cytotoxicity Gene expression profile Mouse liver Nanoparticle Silver 

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Kyungeun Cha
    • 1
  • Hye-Won Hong
    • 1
  • Yeon-Gil Choi
    • 1
  • Min Joo Lee
    • 2
  • Jong Hoon Park
    • 2
  • Hee-Kwon Chae
    • 3
  • Gyuha Ryu
    • 4
  • Heejoon Myung
    • 1
  1. 1.Department of Bioscience and BiotechnologyHankuk University of Foreign StudiesYong-In, Kyung-Gi DoKorea
  2. 2.Department of Biological ScienceSookmyung Women’s UniversitySeoulKorea
  3. 3.Department of Chemistry EducationSeoul National UniversitySeoulKorea
  4. 4.Korea Food and Drug AdministrationSeoulKorea

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