Biotechnology Letters

, Volume 30, Issue 9, pp 1627–1631 | Cite as

Esterification reactions catalyzed by lipases immobilized in organogels: effect of temperature and substrate diffusion

  • M. Zoumpanioti
  • P. Parmaklis
  • P. Domínguez de María
  • H. Stamatis
  • J. V. Sinisterra
  • A. XenakisEmail author
Original Research Paper


Rhizomucor miehei lipase was immobilized in hydroxy(propylmethyl) cellulose or agar gels containing lecithin or AOT microemulsions. The effect of the diffusion of substrates and products to this catalyst was studied, as well as the effect of temperature on the initial rate of ester synthesis. The composition of the gel affects the reaction rate due to mass transport phenomena. The apparent activation energies were higher for the systems based on agar, independently of the microemulsion used, and lower for the systems based on AOT microemulsions, independently of the polymer used.


Activation energy Catalysis Diffusion Lipase Organogels Temperature 



The Greek General Secretariat for Research and Technology is thanked for the financial support (ΠΑΒΕΤ 2006).


  1. Backlund S, Eriksson F, Kanerva L, Rantala M (1995) Selective enzymatic reactions using microemulsion-based gels. Colloids Surf B Biointerfaces 4:121–127CrossRefGoogle Scholar
  2. Biasutti MA, Abuin EB, Silber JJ et al (2008) Kinetics of reactions catalyzed by enzymes in solutions of surfactants. Adv Colloid Interface Sci 136:1–24PubMedCrossRefGoogle Scholar
  3. Blattner C, Zoumpanioti M, Kröner J et al (2006) Biocatalysis using lipase encapsulated in microemulsion-based organogels in supercritical carbon dioxide. J Supercritical Fluids 36:182–193CrossRefGoogle Scholar
  4. Delimitsou C, Zoumpanioti M, Xenakis A, Stamatis H (2002) Activity and stability studies of Mucor miehei lipase immobilized in novel microemulsion-based organogels. Biocatal Biotransform 20(5):319–327CrossRefGoogle Scholar
  5. Fletcher PDI, Freedman RB, Oldfield C, Robinson BH (1985) Activity of lipase in water-in-oil microemulsions. J Chem Soc Faraday Trans I 81:2667–2679CrossRefGoogle Scholar
  6. Hedström G, Backlund S, Eriksson F (2001) Influence of diffusion on the kinetics of an enzyme-catalyzed reaction in gelatine-based gels. J Coll Interface Sci 239:190–195CrossRefGoogle Scholar
  7. Jenta TRJ, Batts G, Rees GD, Robinson BH (1997) Kinetic studies of Chromobacterium viscosum lipase in AOT water in oil microemulsions and gelatin microemulsion-based organogels. Biotechnol Bioeng 54:416–427PubMedCrossRefGoogle Scholar
  8. Nascimento MG, Rezende MC, Vecchia RD et al (1992) Enzyme-catalyzed esterifications in microemulsion-based organo gels. Tetrahedron Lett 33:5891–5894CrossRefGoogle Scholar
  9. Oldfield C (1994) Enzymes in water-in-oil microemulsions (‘reverced micelles’): principles and applications. Biotechnol Genet Eng Rev 12:255–327PubMedGoogle Scholar
  10. Rees GD, Nascimento MG, Jenta T, Robinson BH (1991) Reverse enzyme synthesis in microemulsion-based organo-gels. Biochim Biophys Acta 1073:493–501PubMedGoogle Scholar
  11. Rees GD, Robinson BH (1993) Microemulsions and organogels: properties and novel applications. Adv Mater 5:608–619CrossRefGoogle Scholar
  12. Rees GD, Robinson BH, Stefenson GR (1995) Preparative-scale kinetic resolutions catalysed by microbial lipases immobilised in AOT-stabilised microemulsion-based organogels: cryoenzymology as tool for improving enantioselectivity. Biochim Biophys Acta 1259:73–81PubMedGoogle Scholar
  13. Stamatis H, Xenakis A (1999) Biocatalysis using microemulsion-based polymer gels containing lipase. J Mol Catal B Enzym 6:399–406CrossRefGoogle Scholar
  14. Stamatis H, Xenakis A, Kolisis FN (1999) Bioorganic reactions in microemulsions: the case of lipase. Biotechnol Adv 17:293–318PubMedCrossRefGoogle Scholar
  15. Zoumpanioti M, Karali M, Xenakis A, Stamatis H (2006) Lipase biocatalytic processes in surfactant free microemulsion-like ternary systems and related organogels. Enzyme Microb Technol 39:531–539CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • M. Zoumpanioti
    • 1
  • P. Parmaklis
    • 1
  • P. Domínguez de María
    • 2
  • H. Stamatis
    • 3
  • J. V. Sinisterra
    • 2
  • A. Xenakis
    • 1
    Email author
  1. 1.Institute of Biological Research and Biotechnology National Hellenic Research FoundationAthensGreece
  2. 2.Biotransformations Group, Faculty of PharmacyUniversidad ComplutenseMadridSpain
  3. 3.Biological Applications and Technologies DepartmentUniversity of IoanninaIoanninaGreece

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