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Biotechnology Letters

, Volume 30, Issue 9, pp 1627–1631 | Cite as

Esterification reactions catalyzed by lipases immobilized in organogels: effect of temperature and substrate diffusion

  • M. Zoumpanioti
  • P. Parmaklis
  • P. Domínguez de María
  • H. Stamatis
  • J. V. Sinisterra
  • A. XenakisEmail author
Original Research Paper

Abstract

Rhizomucor miehei lipase was immobilized in hydroxy(propylmethyl) cellulose or agar gels containing lecithin or AOT microemulsions. The effect of the diffusion of substrates and products to this catalyst was studied, as well as the effect of temperature on the initial rate of ester synthesis. The composition of the gel affects the reaction rate due to mass transport phenomena. The apparent activation energies were higher for the systems based on agar, independently of the microemulsion used, and lower for the systems based on AOT microemulsions, independently of the polymer used.

Keywords

Activation energy Catalysis Diffusion Lipase Organogels Temperature 

Notes

Acknowledgement

The Greek General Secretariat for Research and Technology is thanked for the financial support (ΠΑΒΕΤ 2006).

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • M. Zoumpanioti
    • 1
  • P. Parmaklis
    • 1
  • P. Domínguez de María
    • 2
  • H. Stamatis
    • 3
  • J. V. Sinisterra
    • 2
  • A. Xenakis
    • 1
    Email author
  1. 1.Institute of Biological Research and Biotechnology National Hellenic Research FoundationAthensGreece
  2. 2.Biotransformations Group, Faculty of PharmacyUniversidad ComplutenseMadridSpain
  3. 3.Biological Applications and Technologies DepartmentUniversity of IoanninaIoanninaGreece

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