Biotechnology Letters

, Volume 30, Issue 9, pp 1571–1575

Thermal profiling for parallel on-line monitoring of biomass growth in miniature stirred bioreactors

Original Research Paper

Abstract

Recently we have described the design and operation of a miniature bioreactor system in which 4–16 fermentations can be performed (Gill et al., Biochem Eng J 39:164–176, 2008). Here we report on the use of thermal profiling techniques for parallel on-line monitoring of cell growth in these bioreactors based on the natural heat generated by microbial culture. Results show that the integrated heat profile during E. coli TOP10 pQR239 fermentations followed the same pattern as off-line optical density (OD) measurements. The maximum specific growth rates calculated from off-line OD and on-line thermal profiling data were in good agreement, at 0.66 ± 0.04 and 0.69 ± 0.05 h−1 respectively. The combination of a parallel miniature bioreactor system with a non-invasive on-line technique for estimation of culture kinetic parameters provides a valuable approach for the rapid optimisation of microbial fermentation processes.

Keywords

Fermentation heat measurements On-line monitoring Miniature bioreactor Thermal profiling 

Nomenclature

Peh

Power output of the heater (W)

Vch

Voltage supplied to the calibration heater (V)

Rch

Resistance of the calibration heater (Ω)

References

  1. Betts JI, Baganz F (2006) Miniature bioreactors: current practices and future opportunities. Microb Cell Fact 5:1–14CrossRefGoogle Scholar
  2. Bou-Diab A, Schenker B, Marison I, Ampuero S, von Stockar U (2001) Improvements of continuous calibration based on temperature oscillation and application to biochemical reaction calorimetry. Chem Eng 81:113–127CrossRefGoogle Scholar
  3. Doig SD, O’Sullivan LM, Patel S, Ward JM, Woodley JM (2001) Large scale production of cyclohexanone monooxygenase from Escherichia coli TOP10 pQR239. Enzyme Microb Technol 28:265–274PubMedCrossRefGoogle Scholar
  4. Fernandes P, Cabral JMS (2006) Review: microlitre/millilitre shaken bioreactors in fermentative and biotransformation process. Biocatal Biotransformation 24:237–252CrossRefGoogle Scholar
  5. Gill NK, Appleton M, Baganz F, Lye GJ (2008) Design and characterisation of a miniature stirred bioreactor system for parallel microbial fermentations. Biochem Eng J 39:164–176CrossRefGoogle Scholar
  6. Hewitt CJ, Caron G, Nienow AW, McFarlane CM (2000) Use of multi-staining flow cytometry to characterise the physiological state of Escherichia coli W3110 in high cell density fed-batch cultures. Biotechnol Bioeng 63:705–711CrossRefGoogle Scholar
  7. Hüttl R, Harmel J, Lißner A, Wolf G, Klare P, Vonau W, Berthold F, Herrmann S (2008) A small-scale calorimetric reactor system combined with several chemical sensors for the investigation of microbial growth processes. Eng Life Sci 8:56–61CrossRefGoogle Scholar
  8. Jassen M, Patino R, von Stockar U (2005) Application of bench-scale biocalorimetry to photoautotrophic cultures. Thermochim Acta 435:18–27CrossRefGoogle Scholar
  9. Kaiser C, Carvell JP, Luttmann R (2007) A sensitive, compact, in-situ biomass measurement system: controlling and monitoring microbial fermentations using radio-frequency impedance. Bioprocess Int 5:52–56Google Scholar
  10. Lye GJ, Ayazi-Shamlou P, Baganz F, Dalby PA, Woodley JM (2003) Accelerated design of bioconversion processes using automated microscale processing techniques. Trends Biotechnol 21:29–37PubMedCrossRefGoogle Scholar
  11. Marison I, Liu JS, Ampuero S, von Stockar U, Schenker B (1998) Biological reaction calorimetry: development of high sensitivity bio-calorimeters. Thermochim Acta 309:157–173CrossRefGoogle Scholar
  12. Marose S, Lindemann C, Ulber R, Scheper T (1999) Optical sensor systems for bioprocess monitoring. Trends Biotechnol 17:30–34CrossRefGoogle Scholar
  13. Meier-Schneiders M, Schafer F (1996) Quantification of small enthalpic differences in anaerobic microbial metabolism—a calorimetry supported approach. Thermochim Acta 275:1–16CrossRefGoogle Scholar
  14. Meier-Schneiders M, Gosshans U, Busch C, Eigenberger G (1995) Biocalorimetry-supported analysis of fermentation processes. Appl Microbiol Biotechnol 43:431–439Google Scholar
  15. Sarrafzadeh MH, Belloy L, Estaban G, Navarro JM, Ghommidh C (2005) Dielectric monitoring of growth and sporulation of Bacillus thuringiensis. Biotechnol Lett 27:511–517PubMedCrossRefGoogle Scholar
  16. Voisard D, Pugeaud P, Kumar AR, Jenny K, Jayaraman K, Marison IW, von Stockar U (2002) Quantitative calorimetric investigation of fed-batch cultures of Bacillus sphaericus 1593M. Thermochim Acta 394:99–111CrossRefGoogle Scholar
  17. von Stockar U, Marison IW (1989) The use of calorimetry in biotechnology. Adv Biochem Eng Biotechnol 40:93–136Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.The Advanced Centre for Biochemical Engineering, Department of Biochemical EngineeringUniversity College LondonLondonUK
  2. 2.Bioxplore, Unit 9–10BorehamwoodUK

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