Biotechnology Letters

, 30:1469

One-step purification and characterization of an intracellular β-glucosidase from Metschnikowia pulcherrima

  • Paula González-Pombo
  • Gabriel Pérez
  • Francisco Carrau
  • José Manuel Guisán
  • Francisco Batista-Viera
  • Beatriz M. Brena
Original Research Paper

Abstract

A collection of 60 non-Saccharomyces yeasts isolated from grape musts in Uruguayan vineyards was screened for β-glucosidase activity and Metschnikowia pulcherrima was the best source of this enzyme activity. Its major β-glucosidase was successfully purified to homogeneity by ion-exchange chromatography on amino-agarose gel. The enzyme exhibited an optimum catalytic activity at 50°C and pH 4.5 and was active against (1 → 4)-β and (1 → 2)-β glycosidic linkages. In spite of preserving 100% of its activity and stability in the presence of 12% (v/v) ethanol and 5 g glucose/l, the enzyme was unstable below pH 4. We characterized the β-glucosidase from M. pulcherrima with a view to its potential applications in wine-making.

Keywords

β-Glucosidases Enzyme purification Metschnikowia pulcherrima Wine aromatization 

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Paula González-Pombo
    • 1
  • Gabriel Pérez
    • 2
  • Francisco Carrau
    • 2
  • José Manuel Guisán
    • 3
  • Francisco Batista-Viera
    • 1
  • Beatriz M. Brena
    • 1
  1. 1.Departamento de Biociencias, Facultad de QuímicaCátedra de BioquímicaMontevideoUruguay
  2. 2.Departamento de Ciencia y Tecnología de Alimentos, Facultad de QuímicaSección EnologíaMontevideoUruguay
  3. 3.Departamento de Biocatálisis, Instituto de CatálisisCSICMadridSpain

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