Biotechnology Letters

, Volume 30, Issue 8, pp 1359–1365 | Cite as

Heterologous expression of pyruvate decarboxylase in Geobacillus thermoglucosidasius

  • Ann H. Thompson
  • David J. Studholme
  • Edward M. Green
  • David J. Leak
Original Research Paper

Abstract

Expression of a pyruvate decarboxylase (Pdc) pathway in metabolically versatile thermophilic bacteria could create novel ethanologenic organisms, but no suitable thermostable Pdc is available. We have demonstrated that Pdc from Zymomonas mobilis can be expressed in an active form in Geobacillusthermoglucosidasius at up to 52°C, while expression of Pdc polypeptides up to 54°C was evident from Western blotting. By using an unstable lactate dehydrogenase (ldh) mutant of G. thermoglucosidasius, indirect evidence of Pdc activity in vivo was also obtained.

Keywords

Decarboxylase Ethanol Geobacillus Pyruvate Thermophile 

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Ann H. Thompson
    • 1
  • David J. Studholme
    • 1
    • 2
  • Edward M. Green
    • 3
    • 4
  • David J. Leak
    • 1
  1. 1.Division of Biology, Faculty of Natural SciencesImperial College LondonLondonUK
  2. 2.Sainsbury LaboratoryJohn Innes CentreNorwichUK
  3. 3.Green Biologics LtdOxfordshireUK
  4. 4.Agrol LtdSurreyUK

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