Correlation between cell morphology and aggrecan gene expression level during differentiation from mesenchymal stem cells to chondrocytes
- 180 Downloads
A morphological parameter of polygonal index was defined as the ratio of cell adhesion area versus the square of the major cell axis, and cells that had an adhesion area larger than 4000 μm2 and a polygonal index larger than 0.3 were considered large polygonal cells. Cell morphology tended to change from fibroblast-like to polygonal and the percentage of the large polygonal cells increased almost in proportion to aggrecan mRNA expression level during the differentiation culture of mesenchymal stem cells (MSCs) to chondrocytes. Approximately 80% of the large polygonal cells were negative for MSC marker (CD90, CD166) expression and the aggrecan mRNA expression level of the large polygonal cells was markedly higher than that of cells with other morphologies.
KeywordsAggrecan Differentiation Laser Mesenchymal stem cell Morphology Noninvasive
- Hosokawa Y, Takabayashi J, Miura S, Shukunami C, Hiraki Y, Masuhara H (2004) Nondestructive isolation of single cultured animal cells by femtosecond laser-induced shockwave. Appl Phys A79:795–798Google Scholar