Biotechnology Letters

, Volume 30, Issue 7, pp 1189–1195 | Cite as

Correlation between cell morphology and aggrecan gene expression level during differentiation from mesenchymal stem cells to chondrocytes

  • Mutsumi Takagi
  • Takayuki Kitabayashi
  • Satoru Koizumi
  • Haruka Hirose
  • Shin-ichi Kondo
  • Masashi Fujiwara
  • Kosei Ueno
  • Misawa Hiroaki
  • Yoichiroh Hosokawa
  • Hiroshi Masuhara
  • Shigeyuki Wakitani
Original Research Paper

Abstract

A morphological parameter of polygonal index was defined as the ratio of cell adhesion area versus the square of the major cell axis, and cells that had an adhesion area larger than 4000 μm2 and a polygonal index larger than 0.3 were considered large polygonal cells. Cell morphology tended to change from fibroblast-like to polygonal and the percentage of the large polygonal cells increased almost in proportion to aggrecan mRNA expression level during the differentiation culture of mesenchymal stem cells (MSCs) to chondrocytes. Approximately 80% of the large polygonal cells were negative for MSC marker (CD90, CD166) expression and the aggrecan mRNA expression level of the large polygonal cells was markedly higher than that of cells with other morphologies.

Keywords

Aggrecan Differentiation Laser Mesenchymal stem cell Morphology Noninvasive 

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Mutsumi Takagi
    • 1
  • Takayuki Kitabayashi
    • 1
  • Satoru Koizumi
    • 1
  • Haruka Hirose
    • 1
  • Shin-ichi Kondo
    • 1
  • Masashi Fujiwara
    • 1
  • Kosei Ueno
    • 2
  • Misawa Hiroaki
    • 2
  • Yoichiroh Hosokawa
    • 3
  • Hiroshi Masuhara
    • 3
  • Shigeyuki Wakitani
    • 4
  1. 1.Division of Biotechnology and Macromolecular Chemistry, Graduate School of EngineeringHokkaido UniversitySapporoJapan
  2. 2.Section of Informatics and Processing, Research Institute for Electronic ScienceHokkaido UniversitySapporoJapan
  3. 3.Department of Applied Physics, Frontier Research Center and Venture Business LaboratoryOsaka UniversitySuitaJapan
  4. 4.Department of Orthopedic SurgeryOsaka-City University School of MedicineOsakaJapan

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