Biotechnology Letters

, Volume 30, Issue 7, pp 1163–1168

Bone morphogenetic protein-2 enhances bone regeneration mediated by transplantation of osteogenically undifferentiated bone marrow-derived mesenchymal stem cells

Original Research Paper


We have hypothesized that human bone marrow-derived mesenchymal stem cells (BMMSCs), that are not osteogenically differentiated prior to implantation, would regenerate bone extensively in vivo once exogenous bone morphogenetic protein-2 (BMP-2) was delivered to the implantation site. BMP-2 released from heparin-conjugated poly(lactic-co-glycolic acid) (HCPLGA) scaffolds stimulates osteogenic differentiation of cultured BMMSCs. Upon implantation, undifferentiated BMMSCs on BMP-2-loaded HCPLGA scaffolds induce far more extensive bone formation than either undifferentiated BMMSCs or osteogenically differentiated BMMSCs on HCPLGA scaffolds. These BMP-2-loaded HCPLGA scaffolds could prove invaluable for in vivo regeneration of bone from undifferentiated human BMMSCs.


Bone marrow-derived stem cell Bone morphogenetic protein-2 Bone regeneration Tissue engineering 


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Department of Chemical EngineeringHanyang UniversitySeoulKorea
  2. 2.Department of BioengineeringHanyang UniversitySeoulKorea
  3. 3.Department of Orthopaedic Surgery, Ansan Hospital, College of MedicineKorea UniversityAnsanKorea

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