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Biotechnology Letters

, Volume 30, Issue 7, pp 1201–1206 | Cite as

Exopolysaccharides produced by Gordonia alkanivorans enhance bacterial degradation activity for diesel

  • Lin Ta-Chen
  • Jo-Shu Chang
  • Chiu-Chung YoungEmail author
Original Research Paper

Abstract

Exopolysaccharides (EPS) produced by Gordonia alkanivorans CC-JG39 was used to stimulate cell floating, cell growth, and diesel biodegradation of indigenous or commercial-available, diesel-degrading bacteria. Addition of EPS-containing supernatant into the culture medium resulted in floatation of the non-floating bacteria and allowed a 40–45% and 38–42% increase in diesel degradation and cell growth, respectively. The EPS-stimulating effect on cell growth and diesel degradation positively correlated with the EPS dosage. Thus, the EPS may act as a biostimulant for bioremediation of oil-contaminated water or soil.

Keywords

Biostimulation Diesel biodegradation Exopolysaccharides Floatation activity Gordonia alkanivorans 

Notes

Acknowledgment

The authors gratefully acknowledge the financial support from Taiwan’s Ministry of Economic Affairs under Grant no. 95-EC-17-A-10-S1-0013.

References

  1. Abalos A, Vinas M, Sabate J, Manresa MA, Solanas AM (2004) Enhanced biodegradation of Casablanca crude oil by a microbial consortium in presence of a rhamnolipid produced by Pseudomonas aeruginosa AT10. Biodegradation 15:249–260PubMedCrossRefGoogle Scholar
  2. Bai G, Brusseau ML, Miller RM (1997) Biosurefactant-enchanced removal of residual hydrocarbon from soil. J Contam Hydrol 25:157–170CrossRefGoogle Scholar
  3. Bushnell LD, Hass HF (1941) The utilization of certain hydrocarbons by microorganisms. J Bacteriol 41:653–673PubMedGoogle Scholar
  4. Calvo C, Martínez-Checa F, Mota A, Quesada E (1998) Effect of cations, pH and sulfate content on the viscosity and emulsifying activity of the Halomonas eurihalina exopolysaccharide. J Ind Microbiol Biotechnol 20:205–209CrossRefGoogle Scholar
  5. Cooper DG, Goldenberg BG (1987) Surface-active agents from two Bacillus species. Appl Environ Microbiol 53:224–229PubMedGoogle Scholar
  6. Costerton JW (1985) The role of bacterial exopolysaccharides in nature and disease. Dev Ind Microbiol 26:249–261Google Scholar
  7. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356CrossRefGoogle Scholar
  8. Hino S, Watanabe K, Tatkahashi N (1997) Isolation and characterization of slime-producing bacteria capable of utilizing petroleum hydrocarbons as a sole carbon source. J Ferment Bioeng 84:528–531CrossRefGoogle Scholar
  9. Iyer A, Mody K, Jha B (2006) Emulsifying properties of a marine bacterial exopolysaccharide. Enzyme Microb Technol 38:220–222CrossRefGoogle Scholar
  10. Lin TC, Young CC, Ho MJ, Yeh MS, Chou CL, Wei YH, Chang JS (2005) Characterization of floating activity of indigenous diesel-assimilating bacterial isolates. J Biosci Bioeng 99:466–472PubMedCrossRefGoogle Scholar
  11. Martínez-Checa F, Toledo FL, Vilchez R, Quesada E, Calvo C (2002) Yield production, chemical composition, and functional properties of emulsifier H-28 in media containing various hydrocarbons. Appl Microbiol Biotechnol 58:358–363PubMedCrossRefGoogle Scholar
  12. Martínez-Checa F, Toledo FL, Mabrouki KE, Quesada E, Calvo C (2007) Characteristics of bioemulsifer V2-7 synthesized in culture media added of hydrocarbons: chemical composition, emulsifying activity and rheological properties. Bioresour Technol 98:3130–3135PubMedCrossRefGoogle Scholar
  13. Monsigny MC, Petit C, Roche AC (1988) Colorimetric determination of neutral sugars by a resorcinol sulfuric acid micromethod. Anal Chem 175:525–530Google Scholar
  14. Mulligan CN, Yong RN, Gibbs BF (2001) Surfactant-enhanced remediation of contaminated soil: a review. Eng Geol 60:371–380CrossRefGoogle Scholar
  15. Murray RGE, Wood WA, NR Krieg, Gerhardt P (1994) Methods for general and molecular bacteriology. American Society for Microbiology, Washington DC, p 31Google Scholar
  16. Noordman WH, Janssen DB (2002) Rhamnolipid stimulateds uptake of hydrophobic compounds by Pseudomonas aeruginosa. Appl Environ Microbiol 68:4502–4508PubMedCrossRefGoogle Scholar
  17. Ron E, Rosenberg E (2002) Biosurfactants and oil bioremediation. Curr Opin Biotechnol 13:249–252PubMedCrossRefGoogle Scholar
  18. Sutherland IW (1990) Biotechnology of microbial exopolysaccharides. Cambridge University Press, Cambridge, London, p 163Google Scholar
  19. Venosa AD, Zhu X (2003) Biodegradation of crude oil contaminating marine shorelines and freshwater wetlands. Spill Sci Technol Bull 8:163–178CrossRefGoogle Scholar
  20. Wolfaardt GM, Lawrence JR, Headley JV, Robarts RD, Caldwell DE (1994) Microbial exopolymers provide a mechanism for bioaccumulation of contaminants. Microb Ecol 27:279–291CrossRefGoogle Scholar
  21. Wolfaardt GM, Lawrence JR, Robarts RD, Caldwell DE (1998) In situ characterization of biofilm exopolymers involved in the accumulation of chlorinated organics. Microb Ecol 35:213–223PubMedCrossRefGoogle Scholar
  22. Young CC, Lin TC, Yeh MS, Shen FT, Chang JS (2005) Identification and kinetic characteristics of an indigenous diesel-degrading Gordonia alkanivorans strain. World J Microbiol Biotechnol 21:1409–1414CrossRefGoogle Scholar
  23. Zhang Y, Miller RM (1992) Enhanced octadecane dispersion and biodegradation by a Pseudomonas rhamnolipid surfactant (biosurfactant). Appl Environ Microbiol 58:3276–3282PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Sustainable Environment Research CenterNational Cheng Kung UniversityTainanTaiwan
  2. 2.Department of Chemical EngineeringNational Cheng Kung UniversityTainanTaiwan
  3. 3.Department of Soil and Environmental Sciences, College of Agriculture and Natural ResourcesNational Chung Hsing UniversityTaichungTaiwan

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