Biotechnology Letters

, Volume 30, Issue 6, pp 1051–1054 | Cite as

Single bacterial cell detection using a mutant luciferase

  • Kenichi Noda
  • Tadahiro Matsuno
  • Hiroya Fujii
  • Tomonari Kogure
  • Masaaki Urata
  • Yasuo Asami
  • Akio Kuroda
Original Research Paper

Abstract

Previously, we constructed a genetically modified luciferase of Photinus pyralis that generates more than 10-fold higher luminescence intensity than the wild-type enzyme. In this study, we demonstrate that this modified luciferase enables us to detect ATP at 10−18 mol, which is almost equal to the quantity contained in a single bacterial cell. Consequently,we have been able to detect bacterial contamination in samples as low as one colony-forming unit (c.f.u.) for both Escherichia coli and Bacillus subtilis.

Keywords

ATP assay Bacteria detection Mutant firefly luciferase 

References

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Kenichi Noda
    • 1
  • Tadahiro Matsuno
    • 2
  • Hiroya Fujii
    • 2
  • Tomonari Kogure
    • 2
  • Masaaki Urata
    • 1
  • Yasuo Asami
    • 1
  • Akio Kuroda
    • 1
  1. 1.Interdisciplinary Research on Integration of Semiconductor and BiotechnologyHiroshima UniversityHigashihiroshimaJapan
  2. 2.Bussan Nanotech Research InstituteTsukubaJapan

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