Biotechnology Letters

, Volume 30, Issue 5, pp 853–860 | Cite as

Growth phase-dependent changes in the expression of global regulatory genes and associated metabolic pathways in Escherichia coli

  • Mahbuba Rahman
  • Mohammad Rubayet Hasan
  • Kazuyuki ShimizuEmail author
Original Research Paper


Metabolic regulation in Escherichia coli was studied in terms of the changes in the expression of the global regulatory genes rpoD, rpoS, soxRS, cra, fadR, iclR and arcA at three different growth phases, in batch culture. The expression of rpoS and several rpoS-dependent metabolic pathway genes, such as tktB, talA, fumC, acnA, sucA, acs and sodC, were increased (∼1.5 to 2-fold) as the cells entered the late phase of growth. The changes in the expression of other global regulators and their effects on different metabolic pathway genes were less significant, as compared to rpoS, during the later phases of growth.


Batch fermentation Escherichia coli Global regulators Metabolic pathways rpo



We would like to thank Prof. Mori of Keio University, Japan for providing us with the E. coli strain BW25113. Thanks are also due to Brie Fuqua of UC Berkeley, CA, USA for editing the manuscript.


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Mahbuba Rahman
    • 1
  • Mohammad Rubayet Hasan
    • 1
  • Kazuyuki Shimizu
    • 1
    • 2
    Email author
  1. 1.Department of Bioscience and Bioinformatics, Faculty of Computer Science and System EngineeringKyushu Institute of TechnologyFukuokaJapan
  2. 2.Institute for Advanced BiosciencesKeio UniversityTsuruokaJapan

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