Growth phase-dependent changes in the expression of global regulatory genes and associated metabolic pathways in Escherichia coli
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Metabolic regulation in Escherichia coli was studied in terms of the changes in the expression of the global regulatory genes rpoD, rpoS, soxRS, cra, fadR, iclR and arcA at three different growth phases, in batch culture. The expression of rpoS and several rpoS-dependent metabolic pathway genes, such as tktB, talA, fumC, acnA, sucA, acs and sodC, were increased (∼1.5 to 2-fold) as the cells entered the late phase of growth. The changes in the expression of other global regulators and their effects on different metabolic pathway genes were less significant, as compared to rpoS, during the later phases of growth.
KeywordsBatch fermentation Escherichia coli Global regulators Metabolic pathways rpoS
We would like to thank Prof. Mori of Keio University, Japan for providing us with the E. coli strain BW25113. Thanks are also due to Brie Fuqua of UC Berkeley, CA, USA for editing the manuscript.
- Bergmeyer HU (1984) Methods of enzymatic analysis, 3rd edn, vol VI. VCH, WeinheimGoogle Scholar
- Bergmeyer HU (1985) Methods of enzymatic analysis, 3rd edn, vol VII. VCH, WeinheimGoogle Scholar
- Cortay JC, Negre D, Galinier A, Duclos B, Perriere G, Cozzone AJ (1991) Regulation of the acetate operon in Escherichia coli: purification and functional characterization of the IclR repressor. EMBO 10:675–679Google Scholar
- Cronan JE, LaPorte DJ (1996) Tricarboxylic acid cycle and glyoxylate bypass. In: Neidhardt FC, Curtiss R III, Ingraham JL, Lin ECC, Low KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE (eds) Escherichia coli and Salmonella: cellular and molecular biology, 2nd edn. ASM Press, Washington, DC, pp 208–211Google Scholar
- Moat AG, Foster JW, Spector MP (2002) Microbial physiology. John Wiley & Sons, Inc, SingaporeGoogle Scholar
- Rahman M, Hasan MR, Oba T, Shimizu K (2006) Effect of rpoS gene knockout on the metabolism of Escherichia coli during exponential growth phase and early stationary phase based on gene expression, enzyme activities and intracellular metabolite concentrations. Biotechnol Bioeng 94:585–595PubMedCrossRefGoogle Scholar
- Stryer L (1988) Biochemistry. W. H. Freeman and Company, New York, pp 511–512Google Scholar
- Tang Y, Quail MA, Artymiuk PJ, Guest JR, Green J (2002) Escherichia coli aconitases and oxidative stress: post-transcriptional regulation of sodA expression. Microbiol 148:1027–1037Google Scholar