siRNA as a molecular tool for use in Aspergillus niger
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Gene silencing using siRNA has been examined in the industrially-important fungus, Aspergillus niger. Protoplasts of an A. niger strain containing a single genomic copy of the Escherichia coli uidA gene, encoding β-glucuronidase (GUS), under control of the A. niger glaA promoter at the same genomic locus, were exposed to siRNA targeted against the uidA gene. Down-regulation of uidA mRNA and GUS activity by siRNA was observed in mycelia that developed from the protoplasts. The down-regulation was transient and was not carried over to conidiation. We concluded that gene silencing by siRNA provides a relatively quick method for analysis of gene function in A. niger.
KeywordsAspergillus niger Down-regulation GUS Protoplasts siRNA
We acknowledge the financial support of the Biotechnology and Biological Sciences Research Council through the Exploiting Genomics Programme.
- Kariko K, Bhuyan P, Capodici J, Ni HP, Lubinski J, Friedman H, Weissman D (2004) Exogenous siRNA mediates sequence-independent gene suppression by signaling through Toll-like receptor 3. Cells Tissues Organs 117:132–138Google Scholar
- Mouyna I, Henry C, Doering TL, Latge JP (2004) Gene silencing with RNA interference in the human pathogenic fungus Aspergillus fumigatus. FEBS Microbiol Lett 237:317–324Google Scholar