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Biotechnology Letters

, Volume 30, Issue 4, pp 769–774 | Cite as

Characterization of typo-, regio-, and stereo-selectivities of babaco latex lipase in aqueous and organic media

  • E. Cambon
  • J. A. Rodriguez
  • M. Pina
  • V. Arondel
  • F. Carriere
  • F. Turon
  • J. Ruales
  • P. VilleneuveEmail author
Original Research Paper

Abstract

The unripe fruit of babaco (Vasconcellea × heilbornii; syn. Carica pentagona) contains a latex, similar to that in Carica papaya, which exhibits lipolytic activity. Herein, the regioselectivity, stereoselectivity and typoselectivity in both hydrolysis and acyltransfer reactions of babaco latex lipases were studied and compared to those of Carica papaya latex. In hydrolysis, both biocatalysts are 1,3-regioselective with ratios for 1,2-2,3-diacylglycerols/1,3-diacylglycerol of 6.5 and 21 for babaco and papaya, respectively. In contrast, papaya latex had a slight sn-3 stereopreference. Babaco latex displayed a higher activity on triacylglycerols with short chain and unsaturated fatty acids

Keywords

Latex lipase Regioselectivity Stereopreference Typoselectivity 

Notes

Acknowledgements

Jorge Rodriguez is grateful to CONACYT-SFERE program for the financial support of his PhD work.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • E. Cambon
    • 1
  • J. A. Rodriguez
    • 2
  • M. Pina
    • 1
  • V. Arondel
    • 2
  • F. Carriere
    • 2
  • F. Turon
    • 1
  • J. Ruales
    • 3
  • P. Villeneuve
    • 1
    Email author
  1. 1.UMR IATE, Laboratoire de lipotechnieMontpellier cedex 5France
  2. 2.Laboratoire d’Enzymologie Interfaciale et de Physiologie de la Lipolyse, CNRS UPR 9025Marseille cedex 20France
  3. 3.Departamento de ciencia de alimentos y biotechnologia, escuela politecnica nacionalQuitoEcuador

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