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Biotechnology Letters

, Volume 30, Issue 3, pp 541–545 | Cite as

Effective biotic elicitation of Ruta graveolens L. shoot cultures by lysates from Pectobacterium atrosepticum and Bacillus sp.

  • A. Orlita
  • M. Sidwa-Gorycka
  • E. Malinski
  • M. Czerwicka
  • J. Kumirska
  • M. Golebiowski
  • E. Lojkowska
  • P. Stepnowski
Original Research Paper

Abstract

Growth of Ruta graveolens shoots was induced when Bacillus sp. cell lysates were added to the culture medium. Elicitation of coumarin by this lysate was also very effective; the concentrations of isopimpinelin, xanthotoxin and bergapten increased to 610, 2120 and 1460 μg g−1 dry wt, respectively. It also had a significant effect on the production of psoralen and rutamarin (680 and 380 μg g−1 dry wt) and induced the biosynthesis of chalepin, which was not detected in the control sample, up to 47 μg g−1 dry wt With lysates of the Pectobacterium atrosepticum, their effect on growth was not so significant and had no effect on the induction of coumarin accumulation. But elicitation with this lysate was much more effective for inducing the production of furoquinolone alkaloids; the concentrations of γ-fagarine, skimmianine, dictamnine and kokusaginine rose to 99, 680, 172 and 480 μg g−1 dry wt, respectively.

Keywords

Alkaloids Coumarins Elicitors Ruta graveolens L. 

Notes

Acknowledgement

Financial support was provided by the Polish Ministry of Research and Higher Education under grant DS 8200-4-0085-7.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • A. Orlita
    • 1
  • M. Sidwa-Gorycka
    • 2
  • E. Malinski
    • 1
  • M. Czerwicka
    • 1
  • J. Kumirska
    • 1
  • M. Golebiowski
    • 1
  • E. Lojkowska
    • 2
  • P. Stepnowski
    • 1
  1. 1.Faculty of ChemistryUniversity of GdanskGdanskPoland
  2. 2.Intercollegiate Faculty of BiotechnologyUniversity of Gdansk & Medical University of GdańskGdanskPoland

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