Transgenic cotton expressing synthesized scorpion insect toxin AaHIT gene confers enhanced resistance to cotton bollworm (Heliothis armigera) larvae
A synthetic scorpion Hector Insect Toxin (AaHIT) gene, under the control of a CaMV35S promoter, was cloned into cotton via Agrobacterium tumefaciens-mediated transformation. Southern blot analyses indicated that integration of the transgene varied from one to more than three estimated copies per genome; seven homozygous transgenic lines with one copy of the T-DNA insert were then selected by PCR and Southern blot analysis. AaHIT expression was from 0.02 to 0.43% of total soluble protein determined by western blot. These homozygous transgenic lines killed larvae of cotton bollworm (Heliothis armigera) by 44–98%. The AaHIT gene could used therefore an alternative to Bt toxin and proteinase inhibitor genes for producing transgenic cotton crops with effective control of bollworm.
KeywordsAndroctonus australis Bollworm control Cotton (transgenic) Gossypium hirsutum Scorpion toxin in cotton
This study was supported by the grants from the Nature Science Foundation of China (39970416), and the Science and Technology Department of Shanxi Province (041001-1).
- An G (1987) Binary Ti vectors for plant transformation and promoter analysis. Meth Enzymol 153:292–305Google Scholar
- Benedict JH, Altman DW (2001) Commercialization of transgenic cotton expressing insecticidal crystal protein. In: Jenkins JN, Saha S (eds) Genetic improvement of cotton; emerging technologies. Science Publishers, UK, pp 137–201Google Scholar
- Guo HN, Wu JH, Chen XY et al (2003) Cotton plants transformed with the activated chimeric CrylAc and API-B genes. Acta Bot Sin 45:108–113Google Scholar
- Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
- Smith RH, Smith JW, Park SH (2004) Cotton transformation: successes and challenges. In: Liang GH, Skinner DZ (eds) Genetically modified crops, their development, uses and risks. Food Product Press, an Imprint of the Haworth Press. Inc., New York, London, Oxford, pp 247–252Google Scholar