Advertisement

Biotechnology Letters

, Volume 30, Issue 2, pp 263–270 | Cite as

Bioemulsifier production by a halothermophilic Bacillus strain with potential applications in microbially enhanced oil recovery

  • S. M. M. Dastgheib
  • M. A. AmoozegarEmail author
  • E. Elahi
  • S. Asad
  • I. M. Banat
Original Research Paper

Abstract

A halothermotolerant Gram-positive spore-forming bacterium was isolated from petroleum reservoirs in Iran and identified as Bacillus licheniformis sp. strain ACO1 by phenotypic characterization and 16S rRNA analysis. It showed a high capacity for bioemulsifier production and grew up to 60°C with NaCl at 180 g l−1. The optimum NaCl concentration, pH and temperature for bioemulsifier production were 4% (w/v), 8.0, and 45°C, respectively. Although ACO1 did not utilize hydrocarbons, it had a high emulsifying activity (E 24 = 65 ± 5%) on different hydrophobic substrates. Emulsification was optimal while growing on yeast extract as the sole carbon source and NaNO3 as the nitrogen source. The efficiency of the residual oil recovery increased by 22% after in situ growth of B. licheniformis ACO1 in a sand-pack model saturated with liquid paraffin.

Keywords

Biosurfactant Emulsifier Enhanced oil recovery Sand-pack model Thermophile 

References

  1. Bach H, Berdichevsky Y, Gutnick D (2003) An exocellular protein from the oil-degrading microbe Acinetobacter venetianus RAG-1 enhances the emulsifying activity of the polymeric bioemulsifier emulsan. Appl Environ Microbiol 69:2608–2615PubMedCrossRefGoogle Scholar
  2. Bach H, Gutnick DL (2004) Potential applications of bioemulsifiers in the oil industry. In: Vazquez-Duhalt M, Quintero-Ramirez R (eds) Petroleum biotechnology developments and perspectives. Elsevier, Amsterdam, pp 233–281CrossRefGoogle Scholar
  3. Banat IM, Makkar RS, Cameorta SS (2000) Potential commercial applications of microbial surfactants. Appl Microbial Biotechnol. 53:495–508CrossRefGoogle Scholar
  4. Banat IM (1993) The isolation of thermophilic biosurfactant producing Bacillus sp. Biotechnol Lett 15:591–594CrossRefGoogle Scholar
  5. Batista SB, Mounteer AH, Amorim FR, Tótola MR (2006) Isolation and characterization of biosurfactant/bioemulsifier-producing bacteria from petroleum contaminated sites. Bioresour Technol 97:868–875PubMedCrossRefGoogle Scholar
  6. Bodour AA, Maier RM (2002) Biosurfactants: types, screening methods and application. Encyclopedia of environmental microbiology, vol 2. Wiley, New York, pp 750–769Google Scholar
  7. Bognolo G (1999) Biosurfactants as emulsifying agents for hydrocarbons. Colloid Surf Physicochem. Eng Aspect 152:41–52CrossRefGoogle Scholar
  8. Cameotra SS, Makkar RS (1998) Synthesis of biosurfactants in extreme conditions. Appl Microbiol Biotechnol 50:520–529PubMedCrossRefGoogle Scholar
  9. Cooper DG, Goldenberg BG (1987) Surface-active agents from two Bacillus species. Appl Environ Microbiol 53:224–229PubMedGoogle Scholar
  10. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356CrossRefGoogle Scholar
  11. Fujiwara K, Sugai Y, Yazawa N, Ohno K, Hong CX, Enomoto H (2004) Biotechnological approach for development of microbial enhanced oil recovery technique. In: Vazquez-Duhalt M, Quintero-Ramirez R (eds) Petroleum biotechnology developments and perspectives. Elsevier, Amsterdam, pp 233–281Google Scholar
  12. Javaheri M, Jenneman G, Mcinerney MJ, Knapp RM (1985) Anaerobic Production of a Biosurfactant by Bacillus licheniformis JF-2. Appl Environ Microbiol 50:698–700PubMedGoogle Scholar
  13. Jenneman GE, McInerney MJ, Knapp RM, Clark JB, Feero JM, Revus DE, Menzie DE (1983) A halotolerant biosurfactant producing Bacillus specie potential useful for enhanced oil recovery. Dev Ind Microbiol 24:485–492Google Scholar
  14. Kaplan N, Zosim Z, Rosenberg E (1987) Reconstitution of emulsifying activity of Acinetobacter calcoaceticus BD4 emulsan by with pure polysaccharide and protein. Appl Environ Microbiol 53:440–446PubMedGoogle Scholar
  15. Karanth NGK, Deo PG, Veenanadig NK (1999) Microbial production of biosurfactant and their importance. Curr Sci 77:116–126Google Scholar
  16. Lang S, Wagner F (1987) Structure and properties of biosurfactants. In: Kosaric N, Cairns WL, Gray NCC (eds) Biosurfactants and biotechnology. Marcel Dekker, New York, pp 21–45Google Scholar
  17. Luna-Velasco MA, Esparza-Garcia F, Canizares-Villanueva RO, Rodriguez-Vasquez R (2007) Production and properties of a bioemulsifier synthesized by phenanthrene-degrading Penicillim sp. Process Biochem 42:310–314CrossRefGoogle Scholar
  18. Martínez-Checa F, Toledo FL, El Mabrouki K, Quesada E, Calvo C (2007) Characteristics of bioemulsifier V2-7 synthesized in culture media added of hydrocarbons: Chemical composition, emulsifying activity and rheological properties. Bioresour Technol 98:3130–3135PubMedCrossRefGoogle Scholar
  19. Plaza GA, Zjawiony I, Banat IM (2006) Use of different methods for detection of thermophilic biosurfactant producing bacteria from hydrocarbon-contaminated and bioremediated soils. J Pet Sci Eng 50:71–77CrossRefGoogle Scholar
  20. Raymond RL (1961) Microbial oxidation of n-paraffinic hydrocarbons. Develop Ind Microbiol 2:23–32Google Scholar
  21. Rosenberg E, Ron EZ (1999) High- and low-molecular-mass microbial surfactants. Appl Microbiol Biotechnol 52:154–162PubMedCrossRefGoogle Scholar
  22. Shabtai Y, Gutnick DL (1985) Exocellular esterase and emulsan release from the cell surface of Acinetobacter calcoaceticus. J Bacteriol 161:1176–1181PubMedGoogle Scholar
  23. Simbert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. Washington, DC, pp 607–654Google Scholar
  24. Toren A, Orr E, Paitan Y, Ron EZ, Rosenberg E (2002) The active component of the bioemulsifier alasan from Acinetobacter radioresistens KA53 is an OmpA-like protein. J Bacteriol 184:165–170PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • S. M. M. Dastgheib
    • 1
  • M. A. Amoozegar
    • 2
    Email author
  • E. Elahi
    • 3
  • S. Asad
    • 1
  • I. M. Banat
    • 4
  1. 1.Department of Biotechnology, College of ScienceUniversity of TehranTehranIran
  2. 2.Extremophiles Lab, Department of Microbiology, School of Biology, College of ScienceUniversity of TehranTehranIran
  3. 3.Department of Cellular Biology, School of Biology, College of ScienceUniversity of TehranTehranIran
  4. 4.School of Biomedical SciencesUniversity of UlsterColeraineNorthern Ireland, UK

Personalised recommendations