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Biotechnology Letters

, Volume 30, Issue 1, pp 123–126 | Cite as

New isolate of Streptomyces sp. with novel thermoalkalotolerant cellulases

  • Faiez Alani
  • William A. Anderson
  • Murray Moo-Young
Original Research Paper

Abstract

A Streptomyces sp. was isolated that produced novel thermoalkalotolerant cellulase activity after growth on crystalline cellulose at 50°C. Three major components of the cellulases (CMCase, Avicelase and cellobiase) were produced with maximal activities (11.8, 7.8 and 3.9 IU/ml) and maximum specific activities 357, 276 and 118 IU/mg protein, respectively, after 120 h growth. Maximum CMCase activity was between 50 and 60°C measured over 3 h. The enzyme also retained 88% of its maximum activity at 70°C and pH 5, and 80% of the activity at pH 10 and 50°C when assayed after 1 h. After incubation at 40°C for 1 h with commercial detergent (Tide) at pH 11, 95% activity was retained. The enzyme mixture produced glucose from crystalline cellulose.

Keywords

Avicelase Cellobiase CMCase Streptomyces sp 

Notes

Acknowledgments

Portions of this research were funded through the Natural Sciences and Engineering Research Council of Canada.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Faiez Alani
    • 1
  • William A. Anderson
    • 1
  • Murray Moo-Young
    • 1
  1. 1.Biochemical Engineering Group, Department of Chemical EngineeringUniversity of WaterlooWaterlooCanada

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