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Biotechnology Letters

, Volume 30, Issue 1, pp 23–29 | Cite as

Cloning, expression, and pharmacological activity of BmK AS, an active peptide from scorpion Buthus martensii Karsch

  • Jian-Hua Shao
  • Yue-Qiu Wang
  • Xiao-Yan Wu
  • Rui Jiang
  • Rong Zhang
  • Chun-Fu Wu
  • Jing-Hai Zhang
Original Research Paper

Abstract

BmK AS is a β long-chain scorpion peptide from the venom of Buthus martensii Karsch (BmK). It was efficiently expressed as a soluble and functional peptide in Escherichia coli, and purified by metal chelating chromatography. About 4.2 mg/l purified recombinant BmK AS could be obtained. The recombinant BmK AS maintained a similar analgesic activity to the natural one in both the mouse-twisting test and hot-plate procedure. It also exhibited antimicrobial activity against both Gram-positive and Gram-negative bacteria. BmK AS is the first long-chain scorpion peptide reported to have antimicrobial activity, and is a valuable molecular scaffold for pharmacological research.

Keywords

Analgesic activity Antimicrobial activity BmK AS Functional expression Homology model 

Notes

Acknowledgements

Special thanks are due to Liaoning Education Department: education technology investigation supporting project (2004D222).

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Jian-Hua Shao
    • 1
  • Yue-Qiu Wang
    • 1
  • Xiao-Yan Wu
    • 1
  • Rui Jiang
    • 1
  • Rong Zhang
    • 1
  • Chun-Fu Wu
    • 1
  • Jing-Hai Zhang
    • 1
  1. 1.School of Life Science and Bio-pharmaceuticsShenyang Pharmaceutical UniversityShenyangP. R. China

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