Biotechnology Letters

, Volume 29, Issue 12, pp 1857–1863 | Cite as

Growth rate of a non-fermentative Escherichia coli strain is influenced by NAD+ regeneration

  • Consuelo Vázquez-Limón
  • Joel Vega-Badillo
  • Alfredo Martínez
  • Gabriela Espinosa-Molina
  • Guillermo Gosset
  • Xavier Soberón
  • Agustín López-Munguía
  • Joel Osuna
Original Research Paper

Abstract

By complementing a non-fermentative Escherichia coli (ldhApflB) strain with the recombinant Zymomonas mobilis ethanol pathway (pdc, adhB), we evaluated the effect of different levels of enzymatic activity on growth rate demonstrating that there is a direct relationship between anaerobic growth rate and the total specific activity of pyruvate decarboxylase, which is the limiting enzyme of this specific fermentative NAD+ regenerating pathway. This relationship was proved to be useful to establish a selection strategy based on growth rate for the analysis of lctE libraries, which encode lactate dehydrogenase from Bacillus subtilis.

Keywords

Growth rate control Lactate dehydrogenase NAD+ regeneration Non-fermentative Escherichia coli Pyruvate decarboxylase 

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Consuelo Vázquez-Limón
    • 1
  • Joel Vega-Badillo
    • 1
  • Alfredo Martínez
    • 1
  • Gabriela Espinosa-Molina
    • 1
  • Guillermo Gosset
    • 1
  • Xavier Soberón
    • 1
  • Agustín López-Munguía
    • 1
  • Joel Osuna
    • 1
  1. 1.Departamento de Ingeniería Celular y Biocatálisis, Instituto de BiotecnologíaUniversidad Nacional Autónoma de MéxicoCuernavacaMexico

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