Loop-mediated isothermal amplification for rapid detection of Bacillus anthracis spores
- 449 Downloads
A loop-mediated isothermal amplification (LAMP) assay system was employed for detecting Bacillus anthracis spores in pure cultures as well as in various simulated powder samples. The specificity of the designed LAMP primer sets was validated by assaying 13 B. anthracis strains and 33 non-B. anthracis species. The detection limits of the LAMP assay were 10 spores/tube for pure cultures and 100 spores/2 mg powder for simulated powder samples. The results show that the LAMP protocol is a promising method for detecting B. anthracis.
KeywordsBacillusanthracis Detection Isothermal amplification LAMP
This work was supported by the Chinese Academy of Sciences. We thank Dr. Zhiming Yuan for providing Bacillus cereus strains.
- Hoffmaster AR, Meyer RF, Bowen MD, Marston CK, Weyant RS, Thurman K, Messenger SL, Minor EE, Winchell JM, Rassmussen MV, Newton BR, Parker JT, Morrill WE, McKinney N, Barnett GA, Sejvar JJ, Jernigan JA, Perkins BA, Popovic T (2002) Evaluation and validation of a real-time polymerase chain reaction assay for rapid identification of Bacillus anthracis. Emerg Infect Dis 8:1178–1182PubMedGoogle Scholar
- WHO (2003) Guidelines for the surveillance and control of anthrax in humans and animals [monograph on the Internet]. World Health Organization, GenevaGoogle Scholar