Biotechnology Letters

, Volume 29, Issue 12, pp 2001–2005 | Cite as

Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures

  • Hwa-Young Cho
  • Carolyn W. T. Lee-Parsons
  • Sung-Yong H. Yoon
  • Hong Soon Rhee
  • Jong Moon Park
Original Research Paper

Abstract

Production of the benzophenanthridine alkaloids in Eschscholtzia californica suspension cell cultures was optimized by adding 0.5 mg methyl jasmonate (MJ) and 0.02 mg salicylic acid (SA)/g FCW after 7 days cultivation. Sanguinarine reached 24 mg/g DCW by such treatment; 10 times higher than in control cell cultures. MJ and SA induced expression of berberine bridge enzyme and 3′-hydroxy-(S)-N-methylcoclaurine-4′-O-methyltransferase, respectively. MJ plus SA induced over-expression of both enzymes.

Keywords

Benzophenanthridine alkaloid Eschscholtzia californica  Methyl jasmonate Salicylic acid Signal transduction Western blot 

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Hwa-Young Cho
    • 1
  • Carolyn W. T. Lee-Parsons
    • 2
  • Sung-Yong H. Yoon
    • 3
  • Hong Soon Rhee
    • 1
  • Jong Moon Park
    • 1
  1. 1.Advanced Environmental Biotechnology Research Center, School of Environmental Science and Engineering, Department of Chemical EngineeringPohang University of Science and TechnologyHyoja-dongSouth Korea
  2. 2.Chemical Engineering DepartmentNortheastern UniversityBostonUSA
  3. 3.Exelixis Plant SciencesPortlandUSA

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