Biotechnology Letters

, Volume 29, Issue 11, pp 1697–1702 | Cite as

Physical properties and biocompatibility of a porous chitosan-based fiber-reinforced conduit for nerve regeneration

  • Aijun Wang
  • Qiang Ao
  • Yujun Wei
  • Kai Gong
  • Xuesong Liu
  • Nanming Zhao
  • Yandao Gong
  • Xiufang Zhang
Original Research Paper

Abstract

Porous fiber-reinforced chitosan nerve conduits were fabricated from chitosan yarns and a chitosan solution by combining an industrial braiding method with a mold casting/lyophilization technique. The conduits were permeable to molecules ranging in molecular size from 180 Da (glucose) to 66,200 Da (BSA). The compressive load of the reinforced conduits was significantly higher than that of a non-reinforced control conduit at equal levels of strain. The tensile strength of the reinforced conduits was also increased from 0.41 ± 0.17 to 3.69 ± 0.64 MPa. An in vitro cytotoxicity test showed the conduits were not cytotoxic to Neuro-2a cells. Preliminary in vivo implantation testing indicated that the conduits were compatible with the surrounding tissue.

Keywords

Chitosan Fiber Nerve conduit Nerve regeneration 

Notes

Acknowledgments

The authors thank Mr. Bryan Brown for his helpful advice. This work was financially supported by National Basic Research Program of China (also called “973” Program, No. 2005CB623905), the Tsinghua-Yue-Yuen Medical Science Fund, Beijing Municipal Science & Technology Commission (No. H060920050430) and the National Natural Science Foundation of China (No. 30670528).

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Aijun Wang
    • 1
  • Qiang Ao
    • 2
  • Yujun Wei
    • 1
  • Kai Gong
    • 1
  • Xuesong Liu
    • 1
  • Nanming Zhao
    • 3
  • Yandao Gong
    • 1
  • Xiufang Zhang
    • 1
  1. 1.Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane BiotechnologyTsinghua UniversityBeijingChina
  2. 2.Department of Neurosurgery, Yuquan HospitalTsinghua UniversityBeijingChina
  3. 3.Medical SchoolTsinghua UniversityBeijingChina

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