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Biotechnology Letters

, Volume 29, Issue 11, pp 1637–1644 | Cite as

Liposome/DNA systems: correlation between association, hydrophobicity and cell viability

  • Nadia S. Chiaramoni
  • Lucía Speroni
  • María C. Taira
  • Silvia del V. Alonso
Original Research Paper

Abstract

Small unilamellar vesicles associated with plasmid DNA showed maximum association efficiency for a cationic mixture of egg phosphatidylcholine (EPC):1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE):di-1,2-dioleoyl-3-trimethyl ammonium propane (DOTAP) (16:8:1 molar ratio) [65%], followed by neutral lipids EPC:1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE):cholesterol (Chol) (2:2:1 molar ratio) [30%], and a polymerized formulation 1,2-bis(10,12-tricosadiynoyl)sn-glycero-3-phosphocholine (DC8,9PC):DMPE:Chol (2:2:1 molar ratio) [11%]. The hydrophobicity factor (HF) for these formulations followed the trend DC8,9PC:DMPE:CHOL < EPC:DMPE:Chol < EPC:DOPE DOTAP, and DNA association did not alter this trend. Results suggest that the higher the HF value, the more fluid the membrane and the higher the efficiency of DNA association. On the other hand, no differences were observed in cell toxicity with lipids up to 1 mg/ml in VERO cells.

Keywords

Cell viability Hydrophobicity Liposomes Plasmid DNA Polymeric lipid 

Notes

Acknowledgments

We thank Lic. Silvina Mangano, Lic. Ricardo Gargini and Lic. Marcelo Argüelles for their technical assistance in VP7 plasmid DNA obtention and helpful discussions. This work was supported by grants from CIC (Comisión de Investigaciones Científicas), CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas), and the Universidad Nacional de Quilmes (Buenos Aires, Argentina). Silvia del V. Alonso is a Scientific Research Career member of the CONICET, Argentina.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Nadia S. Chiaramoni
    • 1
  • Lucía Speroni
    • 1
  • María C. Taira
    • 1
  • Silvia del V. Alonso
    • 1
  1. 1.Laboratory of Biomembranes (LBM)Universidad Nacional de QuilmesBuenos AiresArgentina

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