Biotechnology Letters

, Volume 29, Issue 10, pp 1575–1582

Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis

  • Patrick C. Y. Woo
  • Shirley S. L. Ma
  • Jade L. L. Teng
  • Maria W. S. Li
  • Susanna K. P. Lau
  • Kwok-yung Yuen
Original Research Paper

Abstract

Among 21 human strains of Laribacter hongkongensis, small plasmids were observed in four strains, and large ones in six strains. The smallest, 3264-bp plasmid, pHLHK19, has only one ORF that encodes a putative replication initiator protein and a predicted origin of replication (ori) with a DnaA box, three 18-bp direct repeats and five pairs of inverted repeats. An Escherichia coliL. hongkongensis shuttle vector was constructed by ligating the HindIII-digested pHLHK19, containing the replication initiator protein and ori of pHLHK19, to HindIII-digested pBK-CMV. This shuttle vector can propagate in E. coli and L. hongkongensis with good transformation efficiencies.

Keywords

Laribacter hongkongensis Plasmid profile Sequence Shuttle vector Transformation 

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Patrick C. Y. Woo
    • 1
    • 2
    • 3
  • Shirley S. L. Ma
    • 3
  • Jade L. L. Teng
    • 3
  • Maria W. S. Li
    • 3
  • Susanna K. P. Lau
    • 1
    • 2
    • 3
  • Kwok-yung Yuen
    • 1
    • 2
    • 3
  1. 1.State Key Laboratory of Emerging Infectious Diseases, Department of MicrobiologyThe University of Hong KongPokfulamHong Kong
  2. 2.Research Centre of Infection and ImmunologyThe University of Hong KongPokfulamHong Kong
  3. 3.Department of MicrobiologyThe University of Hong KongPokfulamHong Kong

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