Biotechnology Letters

, Volume 29, Issue 10, pp 1605–1610

Expression of a ribosome-inactivating protein gene in bitter melon is induced by Sphaerotheca fuliginea and abiotic stimuli

Original Research Paper

Abstract

The gene encoding a single-chain, ribosome-inactivating protein (SCRIP) was cloned from bitter melon (Momordica charantia L.) leaves infected with the fungus, Sphaerotheca fuliginea, by RT-PCR. The ORF was 861 bp. The ribosome-inactivating protein was expressed in E. coli and, when purified, it inhibited the growth of the Sphaerotheca fuliginea in vitro. Northern blot analysis revealed that RIP transcripts rapidly accumulated in leaves 1-day post inoculation with Sphaerotheca fuliginea and reached a peak at 3 d. The expression pattern of RIP induced by methyl jasmonate and salicylic acid were different from that of pathogen-induced expression. Mechanical wounding, silver nitrate and osmotic stress stimulated only a slight accumulation of RIP transcripts. Abscisic acid also induced transcription of RIPs. The signal compounds, ethylene and okadaic acid, induced a moderate accumulation of RIP transcripts.

Keywords

Bitter melon Fungal infection Momordica charantia Ribosome-inactivating protein  Sphaerotheca 

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.State Key Laboratory of Virology, Wuhan Institute of VirologyChinese Academy of SciencesWuhanChina
  2. 2.Graduate School of the Chinese Academy of SciencesBeijingChina
  3. 3.The Laboratory of Toxicology, College of Public HealthZheng Zhou UniversityZheng ZhouChina

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