Biotechnology Letters

, Volume 29, Issue 7, pp 985–994 | Cite as

Enzymatic lysis of microbial cells

Review

Abstract

Cell wall lytic enzymes are valuable tools for the biotechnologist, with many applications in medicine, the food industry, and agriculture, and for recovering of intracellular products from yeast or bacteria. The diversity of potential applications has conducted to the development of lytic enzyme systems with specific characteristics, suitable for satisfying the requirements of each particular application. Since the first time the lytic enzyme of excellence, lysozyme, was discovered, many investigations have contributed to the understanding of the action mechanisms and other basic aspects of these interesting enzymes. Today, recombinant production and protein engineering have improved and expanded the area of potential applications. In this review, some of the recent advances in specific enzyme systems for bacteria and yeast cells rupture and other applications are examined. Emphasis is focused in biotechnological aspects of these enzymes.

Keywords

Bacteriolytic Cell lysis Intracellular protein recovering Yeast-lysing enzyme 

Notes

Acknowledgements

The authors would like to thank the CONICYT (Project 1030797) and the Millennium Scientific Initiative (Millennium Institutes) (ICM-P99-031) for financial support.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Centre for Chemical Engineering and Biotechnology, Department of Chemical Engineering and BiotechnologyUniversity of ChileSantiagoChile

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