Biotechnology Letters

, Volume 29, Issue 5, pp 703–711

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

  • Natalie Muller
  • Madiha Derouazi
  • Frédéric Van Tilborgh
  • Sarah Wulhfard
  • David L. Hacker
  • Martin Jordan
  • Florian M. Wurm
Original Research Paper

DOI: 10.1007/s10529-006-9298-x

Cite this article as:
Muller, N., Derouazi, M., Van Tilborgh, F. et al. Biotechnol Lett (2007) 29: 703. doi:10.1007/s10529-006-9298-x

Abstract

Cell expansion, gene transfer and protein production were all executed with a single serum-free, animal protein-free commercial medium designed for suspension-adapted Chinese hamster ovary cells (CHO DG44). This is a most important process to consider for clinical production of recombinant proteins. The transfection with polyethylenimine (PEI) was shown here to be scalable using both stirred-tank bioreactors of 3- and 150-l and novel agitated cultivation vessels (50 ml ventilated centrifuge tubes and 1-l square-shaped glass bottles) that lack any instrumentation. The transient transfections spanned a range of working volumes from 2 ml to 80 l. The maximum transient recombinant antibody yield was 22 mg/l, the highest ever reported for a multiliter transfection in CHO. The transiently expressed protein had the same extent of glycosylation as the same antibody produced from a stably transfected recombinant CHO cell line.

Keywords

Antibody Bioreactor Orbital shaker Polyethylenimine Recombinant protein Suspension cells 

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Natalie Muller
    • 1
  • Madiha Derouazi
    • 1
    • 2
  • Frédéric Van Tilborgh
    • 1
    • 3
  • Sarah Wulhfard
    • 1
  • David L. Hacker
    • 1
  • Martin Jordan
    • 1
    • 4
  • Florian M. Wurm
    • 1
  1. 1.École Polytechnique Fédérale de Lausanne (EPFL)Institute of Bioengineering, Laboratory of Cellular BiotechnologyLausanneSwitzerland
  2. 2.Department of Biological HaematologyCHU de GRENOBLEGrenoble Cedex 9France
  3. 3.Apoxis S.A., 18 av. de SévelinLausanneSwitzerland
  4. 4.Laboratoires Serono SA – Succursale de Corsier-sur-VeveyRoute de Fenil Z.I. BCorsier-sur-VeveySwitzerland

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