Development of microsatellite markers from tartary buckwheat
- 166 Downloads
A genomic library enriched with (gT)n repeats from tartary buckwheat (Fagopyrum tataricum) was constructed using 5′-anchored PCR for the development of microsattellite markers. Sequencing analysis of 5 clones from the library showed that they all contained microsatellites (totally 10 loci), and each was unique. An additional locus-specific primer was designed according to flanking sequence. Two of the microsatellite loci of 10 tartary buckwheat varieties were amplified using an anchored primer and a locus-specific primer, which revealed a clear polymorphic pattern. The data confirmed that the degenerate primer was reliably anchoring at the 5′-end of the microsatellite, and the primers developed based on this technology could be used for diversity analysis of tartary buckwheat.
KeywordsAnchored PCR Degenerate primer Fagopyrum tataricum Microsatellite Plants diversity
This work was supported by The program of International Plant Genetic Resources Institute (LOAAPO04074) and programs of Science and Technology of Shanxi province, China(041147, 2006031047).
- Alekseeva ES (1986) Selection, cultivation and utilisation of buckwheat. In: Proceedings of the 9th International Symposium on Buckwheat, Pulawy, Poland, 7–12 July 1986Google Scholar
- Gupta PK, Balyan HS, Sharma PC, Ramesh B (1996) Microsatellites in plants: a new class of molecular markers. Curr Sci 70:45–54Google Scholar
- Javornik B, Eggum BO, Kreft I (1981) Studies on protein fractions and protein quality of buckwheat. Genetika 13:115–121Google Scholar
- Schlotterer C, Pemberton J (1994) The use of microsatellites for genetic analysis of natural populations. In: Schierwater B, Streit B, Wagner GP, DeSalle R (eds) Molecular ecology and evolution, approaches and applications. Birkhauser Verlag, Basel, pp. 71–86Google Scholar