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Biotechnology Letters

, Volume 29, Issue 5, pp 823–827 | Cite as

Development of microsatellite markers from tartary buckwheat

  • Yan-Qin LiEmail author
  • Tong-Lin Shi
  • Zong-Wen Zhang
Original Research Paper

Abstract

A genomic library enriched with (gT)n repeats from tartary buckwheat (Fagopyrum tataricum) was constructed using 5′-anchored PCR for the development of microsattellite markers. Sequencing analysis of 5 clones from the library showed that they all contained microsatellites (totally 10 loci), and each was unique. An additional locus-specific primer was designed according to flanking sequence. Two of the microsatellite loci of 10 tartary buckwheat varieties were amplified using an anchored primer and a locus-specific primer, which revealed a clear polymorphic pattern. The data confirmed that the degenerate primer was reliably anchoring at the 5′-end of the microsatellite, and the primers developed based on this technology could be used for diversity analysis of tartary buckwheat.

Keywords

Anchored PCR Degenerate primer Fagopyrum tataricum Microsatellite Plants diversity 

Notes

Acknowledgments

This work was supported by The program of International Plant Genetic Resources Institute (LOAAPO04074) and programs of Science and Technology of Shanxi province, China(041147, 2006031047).

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of BiotechnologyShanxi UniversityTaiyuanP. R. China
  2. 2.IPGRI Office for East Asia, c/o The Chinese Academy of Agricultural SciencesBeijingP. R. China

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