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Biotechnology Letters

, Volume 29, Issue 4, pp 569–574 | Cite as

Production of d-tagatose at high temperatures using immobilized Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana

  • Young-Ho Hong
  • Dong-Woo Lee
  • Sang-Jae Lee
  • Eun-Ah Choe
  • Seong-Bo Kim
  • Yoon-Hee Lee
  • Chan-Ick Cheigh
  • Yu-Ryang PyunEmail author
Original Research Paper

Abstract

Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t 1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.

Keywords

l-Arabinose isomerase Esherichia coli Immobilization d-Tagatose Thermotoga neapolitana 

Notes

Acknowledgements

This work was supported by grant 2001-2-0109 from the Korea Science and Engineering Foundation and grant AIC-08-02 from the Ministry of Commerce, Industry and Energy, Korea.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Young-Ho Hong
    • 1
  • Dong-Woo Lee
    • 2
  • Sang-Jae Lee
    • 1
  • Eun-Ah Choe
    • 3
  • Seong-Bo Kim
    • 4
  • Yoon-Hee Lee
    • 5
  • Chan-Ick Cheigh
    • 1
  • Yu-Ryang Pyun
    • 1
    Email author
  1. 1.Department of BiotechnologyYonsei UniversitySeoulKorea
  2. 2.Department of BiologyUniversity of PennsylvaniaPhiladelphiaUSA
  3. 3.Department of Human GeneticsEmory UniversityAtlantaUSA
  4. 4.R&D Center of Sugar Flour & Edible Oil PlanningCJ Corp.SeoulKorea
  5. 5.Department of Food Science and TechnologyOhio State UniversityColumbusUSA

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