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Biotechnology Letters

, Volume 29, Issue 2, pp 233–236 | Cite as

Optimization of an ethanol production medium in very high gravity fermentation

  • Fan-Qiang Wang
  • Cui-Juan Gao
  • Chun-Yu Yang
  • Ping Xu
Original Research Paper

Abstract

Concentrations of Mg2+, glycine, yeast extract, biotin, acetaldehyde and peptone were optimized by a uniform design process for ethanol production by Saccharomyces cerevisiae. Using non-linear step-wise regression analysis, a predictive mathematical model was established. Concentrations of Mg2+ and peptone were identified as the critical factors: 50 mM Mg2+ and 1.5% (w/v) peptone in the medium increased the final ethanol titre from 14.2% (v/v) to 17% (v/v) in 48 h.

Keywords

Ethanol Fermentation High gravity brewing Saccharomyces cerevisiae 

Notes

Acknowledgement

Author Fan-Qiang Wang would like to acknowledge China Postdoctoral Sciences Foundation for support to complete this study.

References

  1. Alfenore S, Molina-Jouve C, Guillouet SE, Uribelarrea JL, Goma G, Benbadis L (2002) Improving ethanol production and viability of Saccharomyces cerevisiae by a vitamin feeding strategy during fed-batch process. Appl Microbiol Biotechnol 60:67–72PubMedCrossRefGoogle Scholar
  2. Bafrncova P, Smogrovicova D, Slavikova I, Patkova J, Domeny Z (1999) Improvement of very high gravity ethanol fermentation by media supplementation using Saccharomyces cerevisiae. Biotechnol Lett 21:337–341CrossRefGoogle Scholar
  3. Barber AR, Henningsson M, Pamment NB (2002) Acceleration of high gravity yeast fermentations by acetaldehyde addition. Biotechnol Lett 24:891–895CrossRefGoogle Scholar
  4. Cornell NW, Veech RL (1983) Enzymatic measurement of ethanol or NAD in acid extracts of biological samples. Anal Biochem 132:418–423PubMedCrossRefGoogle Scholar
  5. Hu CK, Bai FW, An LJ (2003) Enhancing ethanol tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae by Mg2+ via reduction in plasma membrane permeability. Biotechnol Lett 25:1191–1194PubMedCrossRefGoogle Scholar
  6. Stewart GG, D’Amore T, Panchal CJ, Russell I (1988) Factors that influence the ethanol tolerance of brewer’s yeast strains during high gravity wort fermentation. Tech Q Master Brew Assoc Am 25:47–53Google Scholar
  7. Thomas KC, Hynes SH, Jones AM, Ingledew WM (1993) Production of fuel alcohol from wheat by VHG technology: effect of sugar concentration and fermentation temperature. Appl Biochem Biotechnol 43:211–226CrossRefGoogle Scholar
  8. Thomas KC, Hynes SH, Ingledew WM (1994) Effects of particulate materials and osmoprotectants on very-high-gravity ethanolic fermentation by Saccharomyces cerevisiae. Appl Environ Microbiol 60:1519–1524PubMedGoogle Scholar
  9. Thomas KC, Ingledew WM (1990) Fuel alcohol production: effects of free amino nitrogen on fermentation of very-high-gravity wheat mashes. Appl Environ Microbiol 56:2046–2050PubMedGoogle Scholar
  10. Zhang W, Yu XJ, Yuan Q (1993) Uniform design: a new approach of designing fermentation media. Biotechnol Tech 7:379–384CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Fan-Qiang Wang
    • 1
  • Cui-Juan Gao
    • 1
  • Chun-Yu Yang
    • 1
  • Ping Xu
    • 1
  1. 1.State Key Laboratory of Microbial TechnologyShandong UniversityJinanPeople’s Republic of China

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