Optimization of an ethanol production medium in very high gravity fermentation
Concentrations of Mg2+, glycine, yeast extract, biotin, acetaldehyde and peptone were optimized by a uniform design process for ethanol production by Saccharomyces cerevisiae. Using non-linear step-wise regression analysis, a predictive mathematical model was established. Concentrations of Mg2+ and peptone were identified as the critical factors: 50 mM Mg2+ and 1.5% (w/v) peptone in the medium increased the final ethanol titre from 14.2% (v/v) to 17% (v/v) in 48 h.
KeywordsEthanol Fermentation High gravity brewing Saccharomyces cerevisiae
Author Fan-Qiang Wang would like to acknowledge China Postdoctoral Sciences Foundation for support to complete this study.
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