Biotechnology Letters

, Volume 29, Issue 2, pp 233–236 | Cite as

Optimization of an ethanol production medium in very high gravity fermentation

  • Fan-Qiang Wang
  • Cui-Juan Gao
  • Chun-Yu Yang
  • Ping Xu
Original Research Paper


Concentrations of Mg2+, glycine, yeast extract, biotin, acetaldehyde and peptone were optimized by a uniform design process for ethanol production by Saccharomyces cerevisiae. Using non-linear step-wise regression analysis, a predictive mathematical model was established. Concentrations of Mg2+ and peptone were identified as the critical factors: 50 mM Mg2+ and 1.5% (w/v) peptone in the medium increased the final ethanol titre from 14.2% (v/v) to 17% (v/v) in 48 h.


Ethanol Fermentation High gravity brewing Saccharomyces cerevisiae 



Author Fan-Qiang Wang would like to acknowledge China Postdoctoral Sciences Foundation for support to complete this study.


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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Fan-Qiang Wang
    • 1
  • Cui-Juan Gao
    • 1
  • Chun-Yu Yang
    • 1
  • Ping Xu
    • 1
  1. 1.State Key Laboratory of Microbial TechnologyShandong UniversityJinanPeople’s Republic of China

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