Biotechnology Letters

, Volume 29, Issue 1, pp 57–64 | Cite as

Streptomycin production by Streptomyces griseus can be modulated by a mechanism not associated with change in the adpA component of the A-factor cascade

  • Bin Hong
  • Somkiat Phornphisutthimas
  • Emma Tilley
  • Simon Baumberg
  • Kenneth J. McDowall
Original Research Paper

Abstract

In Streptomyces coelicolor, AtrA is an activator of transcription of the actinorhodin cluster-situated regulator gene actII-ORF4. In previous work, we showed that S. coelicolor AtrA binds in vitro to the promoter of S. griseusstrR, the streptomycin cluster-situated regulator. We show here that S. griseus carries a single close homologue of atrA and that expression of S. coelicolor AtrA in S. griseus causes a DNA binding-dependent reduction in streptomycin production and in the mRNA levels of strR and genes of streptomycin biosynthesis. However, there is no effect on the level of the mRNA of adpA, which is the only transcription factor that has so far been characterised for strR. The adpA gene is directly regulated by ArpA, the receptor protein for the γ-butyrolactone signalling molecule A-factor. Therefore, to our knowledge, our results provide the first in vivo evidence that A-factor-ArpA-AdpA-StrR regulatory cascade represents only part of the full complexity of regulation of streptomycin biosynthesis in S. griseus. The potential biotechnological application of our findings is discussed.

Keywords

Actinorhodin Secondary metabolite production Streptomyces gene regulation S. coelicolor S. griseus Streptomycin 

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Bin Hong
    • 2
  • Somkiat Phornphisutthimas
    • 3
  • Emma Tilley
    • 4
  • Simon Baumberg
    • 1
  • Kenneth J. McDowall
    • 1
  1. 1.Faculty of Biological SciencesUniversity of LeedsLeedsUK
  2. 2.Institute of Medicinal BiotechnologyChinese Academy of Medical SciencesBeijingChina
  3. 3.Institute for Innovation and Development of Learning ProcessMahdiol UniversityBangkokThailand
  4. 4.Institute for Pharmacy and Biomedical SciencesUniversity of StrathclydeGlasgowUK

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