Biotechnology Letters

, Volume 29, Issue 1, pp 137–140

Purification of a trypsin inhibitor from sweet potato in an aqueous two phase system

  • Kuen-Shan Jaw
  • Lin Hung Chou
  • Shiao-Ming Chang
  • Kow-Jen Duan
Original Research Paper

Abstract

Trypsin inhibitor from sweet potato was extracted and purified in a single step using an aqueous two-phase system of polyethylene glycol 6000 (11% w/v), phosphate (16.5% w/v), KCl (9% w/v) and at pH 6. Purity of the trypsin inhibitor was enhanced 3.7-fold, and the recovery was 95%. The purified trypsin inhibitor showed one visible band, and the molecular size was 23 kDa by SDS-PAGE.

Keywords

Aqueous two-phase system Purification Sweet potato Trypsin inhibitor 

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Kuen-Shan Jaw
    • 1
    • 2
  • Lin Hung Chou
    • 2
  • Shiao-Ming Chang
    • 1
  • Kow-Jen Duan
    • 1
  1. 1.Department of BioengineeringTatung UniversityTaipeiTaiwan
  2. 2.Department of Chemical engineeringNorthern Taiwan Institute of Science and TechnologyTaipeiTaiwan

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