Biotechnology Letters

, Volume 29, Issue 1, pp 89–94 | Cite as

Expression of a carbonic anhydrase gene is induced by environmental stresses in Rice (Oryza sativa L.)

  • Song Yu
  • Xinxin Zhang
  • Qingjie Guan
  • Tetsuo Takano
  • Shenkui LiuEmail author
Original Research Paper


Expression of the gene (OsCA1) coding for carbonic anhydrase (CA) in leaves and roots of rice was induced by environmental stresses from salts (NaCl, NaHCO3 and Na2CO3), and osmotic stress (10%, w/v, PEG 6000). CA activity of rice seedlings more than doubled under some of these stresses. Transgenic Arabidopsis over-expressing OsCA1 had a greater salt tolerance at the seedling stage than wild-type plants in 1/2 MS medium with 5 mM NaHCO3, 50 mM NaCl, on 100 mM NaCl. Thus CA expression responds to environmental stresses and is related to stress tolerance in rice.

Key words:

Arabidopsis thaliana Carbonic anhydrase Gene expression Rice (Oryza sativa L.) Stress 



This work was supported by the National High Technology Research and Development Program (863 Program) from the People’s Republic of China, and the Excellent Young Teachers Program in Higher Education Institute MOE, P. R. C.


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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Song Yu
    • 1
  • Xinxin Zhang
    • 1
    • 2
  • Qingjie Guan
    • 1
  • Tetsuo Takano
    • 2
  • Shenkui Liu
    • 1
    Email author
  1. 1.Alkali Soil Natural Environmental Science Center (ASNESC), Stress Molecular Biology LaboratoryNortheast Forestry UniversityHarbinP.R. China
  2. 2.Asian Natural Environment Science Center (ANESC)The University of TokyoNishitokyo, TokyoJapan

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