Expression of a carbonic anhydrase gene is induced by environmental stresses in Rice (Oryza sativa L.)
- 547 Downloads
Expression of the gene (OsCA1) coding for carbonic anhydrase (CA) in leaves and roots of rice was induced by environmental stresses from salts (NaCl, NaHCO3 and Na2CO3), and osmotic stress (10%, w/v, PEG 6000). CA activity of rice seedlings more than doubled under some of these stresses. Transgenic Arabidopsis over-expressing OsCA1 had a greater salt tolerance at the seedling stage than wild-type plants in 1/2 MS medium with 5 mM NaHCO3, 50 mM NaCl, on 100 mM NaCl. Thus CA expression responds to environmental stresses and is related to stress tolerance in rice.
Key words:Arabidopsis thaliana Carbonic anhydrase Gene expression Rice (Oryza sativa L.) Stress
This work was supported by the National High Technology Research and Development Program (863 Program) from the People’s Republic of China, and the Excellent Young Teachers Program in Higher Education Institute MOE, P. R. C.
- Price GD, von Caemmerer S, Evans JR, Yu JW, Lloyd J, Oja V, Kell P, Harrison K, Gallagher A, Badger MR (1994) Specific reduction of chloroplast carbonic anhydrase activity by antisense RNA in transgenic tobacco plants has a minor effect on photosynthesis CO2 assimilation. Planta 193:331–340CrossRefGoogle Scholar
- Wang ZQ, Zhu SQ, Yu RP, Li LQ, Shen GR (1993) The saline-alkali of China. The Science Press 1–305Google Scholar