Biotechnology Letters

, Volume 28, Issue 24, pp 2065–2069 | Cite as

Effect of pretreatment chemicals on xylose fermentation by Pichia stipitis

Original Research Paper
First Online:
Received:
Revised:
Accepted:

Abstract

Pretreatment of biomass with dilute H2SO4 results in residual acid which is neutralized with alkalis such as Ca(OH)2, NaOH and NH4OH. The salt produced after neutralization has an effect on the fermentation of Pichia stipitis. Synthetic media of xylose (60 g total sugar/l) was fermented to ethanol in the presence and absence of the salts using P. stipitis CBS 6054. CaSO4 enhanced growth and xylitol production, but produced the lowest ethanol concentration and yield after 140 h. Na2SO4 inhibited xylitol production, slightly enhanced growth towards the end of fermentation but had no significant effect on xylose consumption and ethanol concentration. (NH4)2SO4 inhibited growth, had no effect on xylitol production, and enhanced xylose consumption and ethanol production.

Keywords

Ammonium sulphate Calcium sulphate Ethanol Pichia stipitis Sodium sulphate Xylose 
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Notes

Acknowledgement

This investigation was supported by the Abengoa-DOE project. We like to thank Dr. Thomas Jeffries for generously providing the yeast strain, David Milam and James Palmer for analytical support on HPLC. We also like to thank Dr. Guillermo Coward-Kelly, Dr. Mads Torry-Smith and Dr. Frank D. Haagensen for useful discussions.

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  1. 1.Novozymes North America Inc.FranklintonUSA

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