Microbial production of 1,3-propanediol by Klebsiella pneumoniae using crude glycerol from biodiesel preparations
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1,3-Propanediol (1,3-PD) was produced by Klebsiella pneumoniae using crude glycerol obtained from biodiesel production. The 1,3-PD concentration of 51.3 g/l−1 on crude glycerol from alkali-catalyzed methanolysis of soybean oil was comparable to that of 53 g/l−1 on crude glycerol derived from a lipase-catalyzed process. The productivities of 1.7 g l−1 h−1 on crude glycerol were comparable to that of 2 g l−1 h−1 on pure glycerol. It could be concluded that the crude glycerol could be directly converted to 1,3-PD without any prior purification.
KeywordsAlkali-catalysis Lipase-catalysis Biodiesel Crude glycerol 1,3-Propanediol
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This work was supported by the China National Natural Science Foundation (grant no. 20576018), the 10th Five-Years’ Projects of China Science and Technology Ministry (2004BA713B06-03), and “973” plan project (2003CB716000).
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