Biotechnology Letters

, Volume 28, Issue 20, pp 1679–1685

A retrovirus-based system to stably silence hepatitis B virus genes by RNA interference

Original Paper


RNA interference (RNAi) might be an efficient antiviral therapy for some obstinate illness. Herein, a retrovirus-based RNAi system was developed to drive expression and delivery of Hepatitis B virus (HBV)-specific short hairpin RNA (shRNA) in HepG2 cells. The levels of HBsAg and HBeAg and that of HBV mRNA were dramatically decreased by this RNAi system in HepG2 cells transfected with Topo-HBV plasmid. Retrovirus-based RNAi thus may be useful for therapy in HBV and other viral infections and provide new clues for prophylactic vaccine development.


Hepatitis B virus HepG2 cells Retrovirus vector RNA interference 


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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  1. 1.School of Life SciencesSichuan University Chengdu China
  2. 2.Molecular Virology Research Center, Institute of MicrobiologyChinese Academy of Sciences Beijing China

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