Biotechnology Letters

, Volume 28, Issue 14, pp 1061–1069 | Cite as

Detection of surface asialoglycoprotein receptor expression in hepatic and extra-hepatic cells using a novel monoclonal antibody

ORIGINAL PAPER
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Abstract

The asialoglycoprotein receptor (ASGPR) is a heterodimeric membrane protein which is involved in the internalization of desialylated glycoproteins and also in the binding and uptake of various pathogenic viruses. To facilitate the analysis of ASGPR expression, we generated a monoclonal antibody, termed ASSA-1, that is specific to the ASGPR H1 subunit based on ELISA and Western blots analysis. ASSA-1 also reacted to surface-displayed ASGPR in live cells thus enabling analysis of ASGPR expression by immunofluorescence flow cytometry, which we used to analyze established human liver cell lines previously confirmed to be positive for ASGPR mRNA expression. In agreement with previous reports, surface ASGPR was also detected in extra-hepatic cells and, surprisingly, even in human T cell lines, which was then further confirmed in activated, but not in resting, primary human peripheral blood lymphocytes. These observations suggest that ASGPR has a broad pattern of expression that even extends into cells from the immune system, which biological meanings still have to be analyzed. We expect that monoclonal antibody ASSA-1 will serve as a new powerful tool in analyzing the biological role of ASGPR in hepatic and extra-hepatic cells.

Keywords

Asialoglycoprotein receptor Monoclonal antibody Flow cytometry T cells Human peripheral blood lymphocyte 
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Notes

Acknowledgements

This study was supported in part of a grant (HSC0120134) from the Ministry of Science and Technology, Korea. The monoclonal antibody ASSA-1 is currently under patent-pending.

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© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  1. 1.Systemic Proteomics Research CenterKorea Research Institute of Bioscience and BiotechnologyYusong, DaejonSouth Korea
  2. 2.Laboratory of Biochemistry, Department of Biological ScienceSungKyunKwan UniversitySuwon, Kyunggi-DoSouth Korea

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