Biotechnology Letters

, Volume 28, Issue 11, pp 815–821 | Cite as

Gene Transfer by DNA/mannosylated Chitosan Complexes into Mouse Peritoneal Macrophages

  • Mayu Hashimoto
  • Minoru Morimoto
  • Hiroyuki Saimoto
  • Yoshihiro Shigemasa
  • Hironobu Yanagie
  • Masazumi Eriguchi
  • Toshinori Sato
Original Paper

Abstract

Chitosan is a biodegradable and biocompatible polymer and is useful as a non-viral vector for gene delivery. In order to deliver pDNA/chitosan complex into macrophages expressing a mannose receptor, mannose-modified chitosan (man-chitosan) was employed. The cellular uptake of pDNA/man-chitosan complexes through mannose recognition was then observed. The pDNA/man-chitosan complexes showed no significant cytotoxicity in mouse peritoneal macrophages, while pDNA/man-PEI complexes showed strong cytotoxicity. The pDNA/man-chitosan complexes showed much higher transfection efficiency than pDNA/chitosan complexes in mouse peritoneal macrophages. Observation with a confocal laser microscope suggested differences in the cellular uptake mechanism between pDNA/chitosan complexes and pDNA/man-chitosan complexes. Mannose receptor-mediated gene transfer thus enhances the transfection efficiency of pDNA/chitosan complexes.

Keywords

Mannose Chitosan Gene transfer Receptor-mediated endocytosis Macrophage Plasmid DNA 

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Mayu Hashimoto
    • 1
  • Minoru Morimoto
    • 2
  • Hiroyuki Saimoto
    • 2
  • Yoshihiro Shigemasa
    • 2
  • Hironobu Yanagie
    • 3
  • Masazumi Eriguchi
    • 3
  • Toshinori Sato
    • 1
  1. 1.Department of Biosciences and InformaticsKeio UniversityYokohamaJapan
  2. 2.Department of Materials ScienceTottori UniversityTottori Japan
  3. 3.Research Center for Advanced Science and TechnologyThe University of TokyoTokyo Japan

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