Many phototrophic microorganisms contain large quantities of high-value products such as n-3 polyunsaturated fatty acids and carotenoids but phototrophic growth is often slow due to light limitation. Some phototrophic microorganisms can also grow on cheap organic substrate heterotrophically. Heterotrophic cultivation can be well controlled and provides the possibility to achieve fast growth and high yield of valuable products on a large scale. Several strategies have been investigated for cultivation of phototrophic microorganisms without light. These include trophic conversion of obligate photoautotrophic microorganisms by genetic engineering, development of efficient cultivation systems and optimization of culture conditions. This paper reviews recent advances in heterotrophic cultivation of phototrophic cells with an emphasis on microalgae.
Keywordsgrowth heterotrophic microalgae phototrophic
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- Brinda, BR, Sarada, R, Kamath, BS, Ravishankar, GA 2004Accumulation of astaxanthin in flagellated cells of Haematococcus pluvialis – cultural and regulatory aspectsCurr. Sci.8712901295Google Scholar
- Chen, F, Johns, MR 1991Effect of C/N ratio and aeration on the fatty acid composition of heterotrophic Chlorella sorokiniana J. Appl. Phycol.3203209Google Scholar
- Cohen Z, Ratledge C (2005) (eds): Single Cell Oils, Champaign, IL, AOCS PressGoogle Scholar
- Swaaf, ME, Pronk, JT, Sijtsma, L 2003aFed-batch cultivation of the docosahexaenoic-acid-producing marine alga Crypthecodinium cohnii on ethanolAppl. Microbiol. Biotechnol.614043Google Scholar
- Jiang, Y, Chen, F 2000aEffects of temperature and temperature shift on docosahexaenoic acid production by the marine microalga Crypthecodinium cohnii J. Am. Oil Chem. Soc.77613617Google Scholar
- Lawlor, DW 2001Photosynthesis3BIOS Scientific Publishers LtdOxfordGoogle Scholar
- Lewin, RA 1962Physiology and Biochemistry of Algae1Academic PressNew YorkGoogle Scholar
- Tripathi, U, Sarada, R, Ravishankar, GA 2002Effect of culture conditions on growth of green alga Haematococcus pluvialis and astaxanthin productionActa Physiol. Plant.24323329Google Scholar
- Vogel, HC, Todaro, CL 1997Fermentation and Biochemical Engineering Handbook: Principles, Process Design, and Equipment2Noyes PublicationsWestwood, New JerseyGoogle Scholar
- Wen ZY (2001) A high yield and productivity strategy for eicosapentaenoic acid production by the diatom Nitzschia laevis in heterotrophic culture. PhD Thesis. Hong Kong: The University of Hong KongGoogle Scholar
- Yaguchi, T, Tanaka, S, Yokochi, T, Nakahara, T, Higashihara, T 1997Production of high yields of docosahexaenoic acid by Schizochytrium sp. strain SR21J. Am. Oil Chem. Soc.7414311434Google Scholar