Biotechnology Letters

, Volume 27, Issue 15, pp 1081–1086 | Cite as

Phenotype of Hepatocyte Spheroids Behavior within Thermo-Sensitive Poly(NiPAAm-co-PEG-g-GRGDS) Hydrogel as a Cell Delivery Vehicle

  • Keun-Hong Park
  • Kun Na
  • Sung Won Kim
  • Sung Yong Jung
  • Kyu Hyung Park
  • Hyung-Min Chung
Article

Abstract

Aggregates (spheroids) of specific cells are often regarded as a better form in artificial organs and mammalian cell bioreactors for improved cell-specific functions. Freshly harvested primary rat hepatocytes, cultivated as spheroids and entrapped in an adhesion molecules of Arg–Gly–Asp (RGD)-conjugated extracellular matrix, have been examined for differentiated morphology and enhanced liver-specific functions. A copolymer of RGD conjugated p(NiPAAm-co-PEG) hydrogel was used to entrap hepatocytes in the forms of spheroids. Over 28 days, entrapped the spheroids had a higher viability and produced albumin and urea at constant rates, while there was slight increase in cell numbers and reduction of albumin secretion in single cell culture in the hydrogel. Hepatocytes cultured in this way are a potentially useful three-dimensional cell system for application in a bioartificial liver device and bioreactor.

Keywords

Arg–Gly–Asp (RGD) artificial liver hepatocytes hydrogel spheroids 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Keun-Hong Park
    • 1
  • Kun Na
    • 2
  • Sung Won Kim
    • 3
  • Sung Yong Jung
    • 1
  • Kyu Hyung Park
    • 1
  • Hyung-Min Chung
    • 1
  1. 1.College of MedicinePochon CHA UniversitySeoulKorea
  2. 2.Division of BiotechnologyThe Catholic University of KoreaBucheonKorea
  3. 3.Biomaterials Research CenterKorea Institute of Science and TechnologySeoulKorea

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