A Novel Oscillating Bioreactor BelloCell: Implications for Insect Cell Culture
and Recombinant Protein Production
BelloCell is a novel packed bed bioreactor that allows alternating nutrient and gas transfer to a culture. Spodoptera frugiperda Sf-9 grown in the BelloCell (300 ml culture) reached 1.3–1.5×107 cells ml−1 in 7–8 days and the total baculovirus-expressed protein yield was 2.3-times that in a stirred tank bioreactor (600 ml culture). The superior cell and protein yields underline the potential of BelloCell for cell culture and recombinant protein production.
Keywordsbaculovirus BelloCell immobilization packed bed reactor protein production
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