Biotechnology Letters

, Volume 27, Issue 15, pp 1059–1065 | Cite as

A Novel Oscillating Bioreactor BelloCell: Implications for Insect Cell Culture and Recombinant Protein Production

  • Jen-Te Lu
  • Yao-Chi Chung
  • Zun-Ren Chan
  • Yu-Chen HuEmail author


BelloCell is a novel packed bed bioreactor that allows alternating nutrient and gas transfer to a culture. Spodoptera frugiperda Sf-9 grown in the BelloCell (300 ml culture) reached 1.3–1.5×107 cells ml−1 in 7–8 days and the total baculovirus-expressed protein yield was 2.3-times that in a stirred tank bioreactor (600 ml culture). The superior cell and protein yields underline the potential of BelloCell for cell culture and recombinant protein production.


baculovirus BelloCell immobilization packed bed reactor protein production 


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Copyright information

© Springer 2005

Authors and Affiliations

  • Jen-Te Lu
    • 1
  • Yao-Chi Chung
    • 1
  • Zun-Ren Chan
    • 1
  • Yu-Chen Hu
    • 1
    Email author
  1. 1.Department of Chemical EngineeringNational Tsing Hua UniversityHsinchuTaiwan

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