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Desalting minimal amounts of DNA for electroporation in E. coli: a comparison of different physical methods

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Abstract

Recovery and purification of ligated plasmid DNA is a critical and limiting step in modern molecular cloning techniques prior to electroporation in E. coli. Four different DNA purification and desalting methods were compared in regard to simple handling, reproducibility, efficiency and cost-effectiveness using picogram and nanogram amounts of DNA. Microcolumn purification of DNA was up to two orders of magnitude more efficient compared to gel filtration, ethanol precipitation or drop dialysis.

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Authors and Affiliations

  1. Institut für Laboratoriumsmedizin und Pathobiochemie, Charité Universitätsmedizin Berlin, Campus Virchow-Klinikum Augustenburger Platz 1, D-13353, Berlin, Germany

    Christina Schlaak, Peter Hoffmann, Karin May & Andreas Weimann

Authors
  1. Christina Schlaak
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  2. Peter Hoffmann
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  3. Karin May
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  4. Andreas Weimann
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Corresponding author

Correspondence to Andreas Weimann.

Additional information

Revisions requested 14 April 2005; Revisions received 10 May 2005

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Schlaak, C., Hoffmann, P., May, K. et al. Desalting minimal amounts of DNA for electroporation in E. coli: a comparison of different physical methods. Biotechnol Lett 27, 1003–1005 (2005). https://doi.org/10.1007/s10529-005-7867-z

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  • DOI: https://doi.org/10.1007/s10529-005-7867-z

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