Biotechnology Letters

, Volume 27, Issue 12, pp 835–838 | Cite as

Ganoderic acid produced from submerged culture of Ganoderma lucidum induces cell cycle arrest and cytotoxicity in human hepatoma cell line BEL7402

Article

Abstract

Ganoderic acid (GA), produced by submerged culture of Ganoderma lucidum, at 500 μg/ml, caused nearly a 70% inhibition of the growth of human hepatoma cell line BEL7402 but not of a normal human liver cell line L02. Flow cytometry analyses showed that GA blocked the BEL7402 cell cycle at the transition from G1 to S phase.

Keywords

cell cycle cytotoxicity Ganoderma lucidum hepatoma submerged culture 

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References

  1. Bao, XF, Wang, XS, Dong, Q, Fang, JN, Li, XY 2002Structural features of immunologically active polysaccharides from Ganoderma lucidumPhytochem59175181CrossRefGoogle Scholar
  2. Fang, QH, Zhong, JJ 2002aEffect of initial pH on production of ganoderic acid and polysaccharide by submerged fermentation of Ganoderma lucidumProc. Biochem37769774CrossRefGoogle Scholar
  3. Fang, QH, Zhong, JJ 2002bSubmerged fermentation of higher fungus Ganoderma lucidum for production of valuable bioactive metabolites-ganoderic acid and polysaccharideBiochem. Eng. J106165CrossRefGoogle Scholar
  4. Hong, KJ, Dunn, DM, Shen, CL, Pence, BC 2004Effects of Ganoderma lucidum on apoptotic and anti-inflammatory function in HT-29 human colonic carcinoma cellsPhytother. Res18768770CrossRefPubMedGoogle Scholar
  5. Hu, H, Ahn, NS, Yang, X, Lee, YS, Kang, KS 2002Ganoderma lucidum extract induces cell cycle arrest and apoptosis in MCF-7 human breast cancer cellInt. J. Cancer102250253CrossRefPubMedGoogle Scholar
  6. Kohda, H, Tokumoto, W, Sakamoto, K, Fuji, M, Hirai, Y, Yamasaki, K, Komoda, Y, Nakamura, H, Ishihara, S, Uchida, M 1985The biologically active constituents of Ganoderma lucidum (Fr.) Karst, Histamine release-inhibitory triterpenesChem. Pharm. Bull3313671374PubMedGoogle Scholar
  7. Komoda, Y, Shimizu, M, Sonoda, Y, Sato, Y 1989Ganoderic acid and its derivatives as cholesterol synthesis inhibitorsChem. Pharm. Bull37531533PubMedGoogle Scholar
  8. Min, BS, Gao, JJ, Hattori, M, Lei, HK, Kim, YH 2001Anticomplement activity of terpenoids from the spores of Ganoderma lucidumPlanta Med67811814CrossRefPubMedGoogle Scholar
  9. Min, BS, Gao, JJ, Nakamura, N, Hattori, M 2000Triterpenes from the spores of Ganoderma lucidum and their cytotoxicity against meth-A and LLC tumor cellsChem. Pharm. Bull4810261033PubMedGoogle Scholar
  10. Min, BS, Nakamura, N, Hattori, M 1998Triterpenes from the spores of Ganoderma lucidum and their inhibitory activity against HIV-1 proteaseChem. Pharm. Bull4616071612PubMedGoogle Scholar
  11. Mizuno, T, Wang, G, Zhang, J, Kawagishi, H, Nishitoba, T, Li, J 1995Reishi, Ganoderma lucidum and Ganoderma tsugae: bioactive substances and medicinal effectsFood Rev. Int11151166Google Scholar
  12. Tang, YJ, Zhong, JJ 2002Fed-batch fermentation of Ganoderma lucidum for hyperproduction of polysaccharide and ganoderic acidEnzyme Microb. Technol312028CrossRefGoogle Scholar
  13. Tsujikura Y, Higuchi T, Miyamoto Y, Sato S, (1992) Manufacture of ganoderic acid by fermentation of Ganoderma lucidum. Jpn. Kokai Tokkyo Koho. JP04304890Google Scholar
  14. Yang, HL, Wu, TX, Zhang, KC 2004Enhancement of mycelial growth and polysaccharide production in Ganoderma lucidum (the Chinese medicinal fungus, ȁ8Lingzhi’) by the addition of ethanolBiotechnol. Lett26841844CrossRefPubMedGoogle Scholar
  15. Zhu, HS, Yang, XL, Wang, LB, Zhao, DX, Chen, L 2000Effects of extracts from sporoderm-broken spores of Ganoderma lucidum on HeLa cellsCell Biol. Toxicol16201206CrossRefPubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.School of Life & Environmental ScienceWenzhou UniversityWenzhouPeople’s Republic of China

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