Kinetic Analysis of the ex vivo Expansion of Human Hematopoietic Stem/Progenitor Cells
The total cell expansion of human umbilical cord blood (CB) and adult bone marrow (BM) CD34+-enriched cells cultured in supplemented serum-free media, either over irradiated human feeder layers or in stroma-free systems, were characterized by a simple kinetic model using only two parameters: the specific cell expansion rate, μ, and the death rate constant, kk. Both CB and BM cells can expand at approximately the same rate (0.21 day−1) in this culture system however, cell death depends on the presence of stroma and the environment in which the cells are cultured.
Keywordscell expansion expansion kinetics model human hematopoietic cells human stem cells stroma
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