Biotechnology Letters

, Volume 28, Issue 5, pp 335–340 | Cite as

Kinetic Analysis of the ex vivo Expansion of Human Hematopoietic Stem/Progenitor Cells

  • Raquel Gonçalves
  • Cláudia Lobato da Silva
  • Bruno S. Ferreira
  • Maria Amélia N. D. A. Lemos
  • Francisco Lemos
  • Graça Almeida-Porada
  • Joaquim M. S. Cabral
Article

Abstract

The total cell expansion of human umbilical cord blood (CB) and adult bone marrow (BM) CD34+-enriched cells cultured in supplemented serum-free media, either over irradiated human feeder layers or in stroma-free systems, were characterized by a simple kinetic model using only two parameters: the specific cell expansion rate, μ, and the death rate constant, kk. Both CB and BM cells can expand at approximately the same rate (0.21 day−1) in this culture system however, cell death depends on the presence of stroma and the environment in which the cells are cultured.

Keywords

cell expansion expansion kinetics model human hematopoietic cells human stem cells stroma 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Berenson, RJ, Andrews, RG, Bensinger, WI, Kalamasz, D, Knitter, G, Buckner, CD, Bernstein, ID 1988Antigen CD34+ marrow cells engraft lethally irradiated baboonsJ. Clin. Invest.81951955PubMedGoogle Scholar
  2. Cabrita, G, Ferreira, BS, Lobato da Silva, C, Gonçalves, R, Almeida-Porada, G, Cabral, JMS 2003Hematopoietic stem cells: from the bone to the bioreactorTrends Biotechnol.21233240CrossRefPubMedGoogle Scholar
  3. Emerson, SG 1996Ex vivo expansion of hematopoietic precursors, progenitors, and stem cells: the next generation of cellular therapeuticsBlood8730823088PubMedGoogle Scholar
  4. Lewis, ID, Almeida-Porada, G, Du, J, Lemischka, IR, Moore, KA, Zanjani, ED, Verfaillie, CM 2001Umbilical cord blood cells capable of engrafting in primary, secondary, and tertiary xenogeneic hosts are preserved after ex vivo culture in a noncontact systemBlood9734413449PubMedGoogle Scholar
  5. Lobato da Silva, C, Gonçalves, R, Lemos, F, Lemos, MANDA, Zanjani, ED, Almeida-Porada, G, Cabral, JMS 2003Modelling of ex vivo expansion/maintenance of hematopoietic stem cellsBioproc. Biosyst. Eng.23365369Google Scholar
  6. Lobato da Silva, C, Gonçalves, R, Crapnell, KB, Cabral, JMS, Zanjani, ED, Almeida-Porada, G. 2005A human stromal-based serum free culture system supports the ex vivo expansion/maintenance of bone marrow and cord blood hematopoietic stem/progenitor cellsExp. Hematol.33828835Google Scholar
  7. Majumdar, M, Thiede, M, Mosca, J, Moorman, M, Gerson, L 1998Phenotypic and functional comparison of cultures of marrow-derived mesenchymal stem cells (MSCs) and stromal cellsJ. Cell. Physiol.1765766CrossRefPubMedGoogle Scholar
  8. McAdams, TA, Miller, WM, Papoutsakis, ET 1996Hematopoietic cell culture therapies (Part I): cell culture considerationsTrends Biotechnol.14341349CrossRefPubMedGoogle Scholar
  9. Moore, MA 2005Converging pathways in leukemogenesis and stem cell self-renewalExp. Hematol.33719737CrossRefPubMedGoogle Scholar
  10. Press, WH, Teukolsky, SA, Vetterling, WT, Flannery, BP 1992Numerical Recipes in Fortran – the Art of Scientific Computing2Cambridge University PressCambridgeGoogle Scholar
  11. Shih, CC, Hu, MC, Hu, J, Medeiros, J, Forman, SJ 1999Long-term ex vivo maintenance and expansion of transplantable human hematopoietic stem cellsBlood9416231636PubMedGoogle Scholar
  12. Stein, MI, Zhu, J, Emerson, SG 2004Pathways regulating the self-renewal of hematopoietic stem cellsExp. Hematol.3211291136CrossRefPubMedGoogle Scholar
  13. Yoo, ES, Lee, KE, Seo, JW, Yoo, EH, Lee, MA, Im, SA, Mun, YC, Lee, SN, Huh, JW, Kim, MJ, Jo, DY, Ahn, JY, Lee, SM, Chung, WS, Kim, JH, Seong, CM 2003Adherent cells generated during long-term culture of human umbilical cord blood CD34+ cells have characteristics of endothelial cells and beneficial effect on cord blood ex vivo expansionStem Cells21228235CrossRefPubMedGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • Raquel Gonçalves
    • 1
    • 2
  • Cláudia Lobato da Silva
    • 1
    • 2
  • Bruno S. Ferreira
    • 1
  • Maria Amélia N. D. A. Lemos
    • 1
  • Francisco Lemos
    • 1
  • Graça Almeida-Porada
    • 1
  • Joaquim M. S. Cabral
    • 1
  1. 1.Centro de Engenharia Biológica e QuímicaInstituto Superior TécnicoLisboaPortugal
  2. 2.Department of Animal BiotechnologyUniversity of NevadaRenoUSA

Personalised recommendations