Advertisement

Biotechnology Letters

, Volume 28, Issue 1, pp 55–59 | Cite as

Extremely Rapid Extraction of DNA from Bacteria and Yeasts

  • Hai-Rong Cheng
  • Ning Jiang
Article

Abstract

A very simple and rapid method for extracting genomic DNA from Gram-negative bacteria, Gram-positive bacteria and yeasts is presented. In this method, bacteria or yeasts are lysed directly by phenol and the supernatant is extracted with chloroform to remove traces of phenol. The supernatant contains DNA that is suitable for molecular analyses, such as PCR, restriction enzyme digestion and genomic library construction. This method is reproducible and simple for the routine DNA extraction from bacteria and yeasts.

Keywords

bacteria DNA extraction yeast 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Becker, A, Katzen, F, Puhler, A, Ielpi, L 1998Xanthan gum biosynthesis and application: a biochemical/genetic perspectiveAppl. Microbiol. Biotechnol.50145152PubMedCrossRefGoogle Scholar
  2. Flamm, RK, Hinrichs, DJ, Thomashow, MF 1984Introduction of pAMβ1 into Listeria monocytogenes by conjugation and homology between native L. monocytogenes plasmidsInfect. Immun.44157161PubMedGoogle Scholar
  3. Lee, YK, Kim, HW, Liu, CL, Lee, HK 2003A simple method for DNA extraction from marine bacteria that produce extracellular materialsJ. Microbiol. Methods52245250PubMedCrossRefGoogle Scholar
  4. Müller, FM, Werner, KE, Kasai, M, Francesconi, A, Chanock, SJ, Walsh, TJ 1998Rapid extraction of genomic DNA from medically important yeasts and filamentous fungi by high-speed cell disruptionJ. Clin. Microbiol.3616251629PubMedGoogle Scholar
  5. Neumann, B, Pospiech, A, Schairrer, HU 1992Rapid isolation of genomic DNA from Gram-negative bacteriaTrends Genet.8332333PubMedGoogle Scholar
  6. Niemi, RM, Heiskanen, I, Wallenius, K, Lindstrom, K 2001Extraction and purification of DNA in rhizosphere soil samples for PCR-DGGE analysis of bacterial consortiaJ. Microbiol. Methods45155165Google Scholar
  7. Sambrook, J, Russell, DW 2001Rapid isolation of yeast DNASambrook, JRussell, DW eds. Molecular Cloning, a Laboratory ManualCold Spring Harbor Laboratory PressNew York631632Google Scholar
  8. Syn, CK, Swarup, S 2000A scalable protocol for the isolation of large-sized genomic DNA within an hour from several bacteriaAnal. Biochem.2788690PubMedCrossRefGoogle Scholar
  9. Wilson, K 1990Preparation of genomic DNA from bacteriaAusubel, FMBrent, R eds. Current Protocols in Molecular BiologyGreene Publ. Assoc. and Wiley InterscienceNew York241245Google Scholar
  10. Xue, Y, Xu, Y, Liu, Y, Ma, Y, Zhou, P 2001Thermoanaerobacter tengcongensis sp. nov., a novel anaerobic, saccharolytic, thermophilic bacterium isolated from a hot spring in Tengcong, ChinaInt. J. Syst. Evol. Microbiol.5113351341PubMedGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Center for Microbial Biotechnology, Institute of Microbiology, Chinese Academy of SciencesBeijingChina
  2. 2.Graduate School, Chinese Academy of SciencesBeijingChina

Personalised recommendations