Biotechnology Letters

, Volume 27, Issue 18, pp 1381–1386

Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from Gluconate and Glucose by Recombinant Aeromonas hydrophila and Pseudomonas putida

  • Yuan-Zheng Qiu
  • Jing Han
  • Jing-Jing Guo
  • Guo-Qiang Chen
Article

DOI: 10.1007/s10529-005-3685-6

Cite this article as:
Qiu, YZ., Han, J., Guo, JJ. et al. Biotechnol Lett (2005) 27: 1381. doi:10.1007/s10529-005-3685-6

Abstract

Aeromonas hydrophila 4AK4 and Pseudomonas putida GPp104 were genetically engineered to synthesize poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) using gluconate and glucose rather than fatty acids. A truncated tesA gene, encoding cytosolic thioesterase I of Escherichia coli which catalyzes the conversion of acyl-ACP into free fatty acids, was introduced into A. hydrophila 4AK4. When grown in gluconate, the recombinant A. hydrophila 4AK4 synthesized 10% (w/w) PHBHHx containing 14% (mol/mol) 3-hydroxyhexanoate. If additional PHBHHx synthesis genes, phaPCJ, were over-expressed with the truncated tesA in A. hydrophila 4AK4, the PHBHHx content increased to 15% (w/w) and contained 19% (mol/mol) 3-hydroxyhexanoate. Recombinant P. putida GPp104 harboring phaC encoding PHBHHx synthase of A. hydrophila, phaB encoding acetoacetyl-CoA reductase of Wautersia eutropha and phaG encoding 3-hydroxyacyl-ACP-CoA transferase of P. putida, synthesized 19% (w/w) PHBHHx containing 5% (mol/mol) 3-hydroxyhexanoate from glucose. The results suggest that the engineered pathways were applicable to synthesize PHBHHx from unrelated carbon sources such as gluconate and glucose.

Key words

Aeromonas hydrophila gluconate glucose poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) polyhydroxyalkanoates Pseudomonas putida 

Copyright information

© Springer 2005

Authors and Affiliations

  • Yuan-Zheng Qiu
    • 1
  • Jing Han
    • 2
  • Jing-Jing Guo
    • 1
  • Guo-Qiang Chen
    • 1
  1. 1.Department of Biological Sciences and BiotechnologyTsinghua University BeijingChina
  2. 2.School of Life ScienceShandong Normal UniversityJinanChina

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