Biotechnology Letters

, Volume 27, Issue 9, pp 623–628 | Cite as

GABAA receptor-associated protein (GABARAP) induces apoptosis by interacting with DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47)

Article

Abstract

GABAA receptor-associated protein (GABARAP) is a 14-kDa cytoplasmic protein initially identified as a molecular chaperone for GABAA receptor in cortical neurons. However, evidence indicates that the function of GABARAP is much broader than a specific role in neuronal cells. As an initial step to define the biological role of GABARAP, we searched for binding partners of GABARAP using co-immunoprecipitation coupled with LC-MS/MS analysis. As a result, DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47), recently identified as RNA helicase, is identified as a binding partner of GABARAP. An interaction between GABARAP and DDX 47 was further confirmed by yeast two-hybrid systems. Further, co-transfection of GABARAP and DDX47 cDNA into a tumor cell line induces apoptosis. This result may be the first evidence showing that GABARAP and DDX 47 are involved in the apoptotic process.

Keywords

apoptosis DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47) GABAA receptor-associated protein (GABARAP) RNA helicase 

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Notes

Acknowledgement

This work was supported by the research fund of Hanyang University (HY-2004-S).

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Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Department of Microbiology and Immunology, School of MedicineHanyang UniversitySung-Dong KuSouth Korea
  2. 2.Department of Obstetrics and GynecologyChonbuk National University Medical SchoolDukjin KuSouth Korea
  3. 3.Molecular Therapy Research CenterSungkyunkwan University, Samsung Medical Center Annex 8FKangnam KuSouth Korea

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